摘要
以海南龙血树优株顶芽和茎段为外植体,通过不同细胞分裂素、生长素以及培养基不同浓度的组合对诱导芽的分化、增殖、伸长及生根的对比实验及试管苗移栽管理,筛选出MS+蔗糖30 g/L+6-BA 3．0 mg/L+NAA 0．01 mg/L培养基诱导芽的形成,MS+蔗糖30 g/L+6-BA 2．0 mg/L+NAA 0．01 mg/L培养基用于芽的增殖,MS+蔗糖30 g/L+6-BA 0．5 mg/L培养基用于壮芽伸长;1/2MS+蔗糖15 g/L+NAA 0．2 mg/L用于诱导芽生根;选用泥炭土、椰糠和珍珠岩混合基质,于晚春和秋季移栽试管苗,成活率达90％左右,达到工厂化苗木生产的要求。
The excellent plant apical bud and stem were used as explant in some contrast experiment about Induction of bud differentiation, elongation, multiplication and radication with different concentrations of auxetic, auxin, various combination of culture medium concentration and transplant survivable ratio of the tube seedling in different moisture, temperature and medium. The results show that ： the culture medium of MS ＋ 30 g/1 sucrose ＋ 6- BA 3.0 mg/L ＋ NAA O. Ol mg/L is suitable for the induction of bud formation, MS ＋ 30 g/Lsucrose ＋ 6-BA 2.0 mg/L ＋ NAA O. 02 mg/L can be applied in bud multiplication, MS ＋ 30 g/L sucrose ＋ 6-BA O. 5 mg/L is fit for robust bud elongation, and the suitable medium for bud radication induction is 1/2MS ＋ 15 g/L sucrose ＋ NAA O. 2 mg/L. The application of compound medium of peat soil, coco - husk and perlite powder in tub seedling transplant in late spring and autum will make the transplant surviable ratio come to about 90% that is up to the deamand of the seedlings industrial production.
出处
《广东林业科技》
2006年第1期22-25,共4页
Forestry Science and Technology of Guangdong Province
关键词
海南龙血树组织培养培养基
Dracaena cambodinna, tissue culture, culture medium
