摘要
目的探讨苦参素在诱导肝星状细胞HSC-T6凋亡过程中的作用,以及对端粒酶及其亚单位端粒酶逆转录酶(rTERT)mRNA表达的影响。方法将不同质量浓度的苦参素与HSC-T6细胞共培养不同时间后,MTT法检测苦参素对HSC-T6细胞的生长抑制作用;流式细胞术检测苦参素对HSC-T6细胞凋亡的影响;TRAP-PAGE-银染法检测苦参素作用后HSC-T6细胞中端粒酶活性的改变;RT-PCR检测苦参素对HSC-T6细胞rTERT mRNA表达的影响。结果苦参素能显著抑制HSC-T6细胞生长、诱导细胞凋亡、降低端粒酶活性,并抑制rTERT mRNA表达。结论苦参素对HSC-T6细胞增殖具有抑制作用,可能与抑制细胞端粒酶及其亚单位rTERT的活性有关。
Objective To investigate the induction effect ofoxymatrine on the apoptosis process in rat hepatic stellate cell line HSC-T6 and to define its impact on telomerase activity and mRNA expression of subunit telomerase reverse transcriptase （rTERT）. Methods HSC-T6 cells were cultivated with different concentration of oxymatrine for different time periods. Effect of oxymatrine on the growth inhibition of HSC-T6 cells was analyzed by MTT assay. Apoptosis of HSC-T6 cells was detected by flow cytometry. Telomerase activity was determined by TRAP-PAGE-silver staining and the expression ofrTERT mRNA was examined by RT-PCR assay. Results Oxymatrine significantly suppressed the growth of HSC-T6 cells and induced apoptosis, and also reduced the activity of telomerase and inhibited the rTERT-mRNA expression in HSC-T6 cells. Conclusion The function that oxymatrine inhibits the proliferation of HSC-T6 cells may be associated with its action on the cellular telomerase and telomerase rTERT-mRNA activity.
出处
《中草药》
CAS
CSCD
北大核心
2014年第14期2051-2055,共5页
Chinese Traditional and Herbal Drugs
基金
湖南省卫生厅科研基金课题(132011-037)
