摘要
目的:建立外周血端粒酶逆转录酶(hTERT)mRNA表达量检测的实时荧光定量逆转录聚合酶链反应(RT-PCR)系统,检测结直肠癌患者hTERT mRNA的表达并探讨其对结直肠癌微转移的诊断价值.方法:用实时荧光定量RT-PCR技术检测53例结直肠癌患者和21名健康人的外周血标本hTERT mRNA表达情况,分析其表达与肿瘤临床病理特征的关系并应用接受者操作特征曲线(ROC曲线)分析hTERT mRNA检测对结直肠癌的诊断价值.结果:结直肠癌组外周血hTERT mRNA表达水平显著高于正常对照组(t'=7.953,P<0.05),其表达与肿瘤淋巴结转移、血行转移和TNM分期相关(t'/t=2.334,2.149,2.460,均P<0.05).hTERT mRNA诊断结直肠癌的ROC曲线下面积0.91,诊断界值为Ct≤32.结论:应用实时荧光定量RT-PCR技术克服了传统PCR只能定性检测而不能定量检测的缺点;外周血hTERT mRNA可作为诊断结直肠癌微转移的标记物.
AIM： To establish a real-time fluorescent quantitative reverse transcriptase polymerase chain reaction （RT-PCR） system for determining the expression of human telomerase reverse transcriptase （hTERT） mRNA in colorectal cancer （CRC）, and to evaluate their diagnostic value for micrometastasis of CRC.
METHODS： The expression of hTERT mRNA in 53 CRC patients and 21 healthy subjects was determined using real-time fluorescent quantitative RT-PCR. Correlations between their expression and clinical pathological characteristics of CRC were analyzed. Receiver operating characteristic curve （ROC curve） was used to evaluate the diagnostic value for micrometastasis of CRC.
RESULTS： The expression levels of peripheral blood hTERT mRNA were significantly higher in CRC patients than in the normal controls （t′= 7.953, P 〈 0.05）, and their expressions were correlated with lymph node metastasis, hematogenous metastasis and TNM staging of CRC （t′/t = 2.334, 2.149, 2.460, all P 〈 0.05）. The area under ROC curve of hTERT mRNA in diagnosing CRC was 0.91, and the cutoff value was Ct≤32.
CONCLUSION： Real-time fluorescent quantitative RT-PCR has an advantage of giving the quantitative expression of hTERT mRNA compared with the traditional PCR which was only applied to the qualitative expression. Peripheral blood hTERT mRNA is a novel and excellent marker for micrometastasis of CRC.
出处
《世界华人消化杂志》
CAS
北大核心
2008年第36期4066-4070,共5页
World Chinese Journal of Digestology
基金
青岛市科技计划资助项目
No.kzd-19~~
