摘要
对江西铅山红芽芋(Colocasia esculenta var.cormosus cv.Hongyayu)胚性愈伤组织包埋干燥法超低温保存进行了初步的研究。结果表明:江西铅山红芽芋胚性愈伤组织包埋干燥法超低温保存较佳的条件为:0.75 mol·L-1蔗糖预培养3 d;脱水方式为空气干燥7 h或硅胶干燥11 h;化冻温度为37℃(2 min);冻后培养条件为暗培养7 d再转到光周期中培养。此方法超低温保存后的平均成活率约为45%。超低温保存时间以及是否去除包裹的褐藻酸钙对其成活率无显著性影响。形态学和细胞学检测表明红芽芋胚性愈伤组织冻后再生苗与母本材料相比没有发生变异。
Cryopreservation of Jiangxi Yanshan red bud taro （Colocasia esculenta var. cormosus cv. Hongyayu） embryogenic calli by encapsulation-dehydration was studied. The results showed that the optimal preculture condition of cryopreservation by encapsulation-dehydration was precultured on MS medium supplemented with 0. 75 mol. L-1 sucrose for 3 days, The optimal dehydration method was dehydration by sterile air in a laminar flow hood for 7 h or ster- ile dry silica gel for 11 h. the optimal thawing temperature was 37 ℃ （2 min）. The optimal culture condition after eryopreservation was first in the dark for 7 d and then transferred to the photoperiod of 14 h · d-l. The average survival rate of embryogenic calli after cryopreservation by encapsulation-dehydration was about 45%. Cryopreservation time and whether the removal of encapsulated calcium alginate had no significant impact on the survival rate. Morphological and cytological study demonstrated that the regenerants were genetically and morphological stable.
出处
《植物分类与资源学报》
CAS
CSCD
北大核心
2014年第4期505-512,共8页
Plant Diversity
基金
江西省科技厅2012年农业科技支撑项目基金(20122BBF60126)
2013年度江西省高等学校科技落地计划项目(KJLD13088)
关键词
江西铅山红芽芋
胚性愈伤组织
包埋干燥法
超低温保存
Red bud taro （Colocasia esculenta var. cormosus cv. Hongyayu）
Embryogenic callus
Encapsulation-dehydration
Cryopreservation
