摘要
用包埋脱水法冰冻保存绿色巴夫藻 (Pavlovaviridis)、湛江等鞭金藻 (Isochrysiszhanjian gensis)和球等鞭金藻 (Isochrysisgalbana 30 1 1 )等三种海洋饵料金藻 ,探讨了脱水速率、胶球含水量以及化冻后恢复方法对冰冻保存存活率的影响。结果表明 ,三种藻都在 - 0 9%含水量 /h的平均脱水速率下获得最高存活率。各种藻在冰冻前的胶球最佳含水量不同 ,绿色巴夫藻为35 % ,湛江等鞭金藻和球等鞭金藻都为 30 %。化冻后 ,含绿色巴夫藻的胶球在培养基中 2 2℃暗放置 48h存活率最高 ;另两种藻在相对湿度为 75 %的气相中 2 2℃暗放置 1 2h存活率最高。在本实验条件下 ,绿色巴夫藻、湛江等鞭金藻和球等鞭金藻的冰冻保存的存活率可分别达到74%、1 5 %和 1 7%。
Three golden-brown algae (Chrysophyceae), Pavlova viridis, Isochrysis zhanjiangensis and I. galbana 3011 used as aquacultural diets, were preserved in liquid nitrogen using encapsulation-dehydration. Algal cells in the early stationary phase were used for cryopreservation. Harvested cells were encapsulated in 3% Ca-alginate beads with 30‰ NaCl, about 2 million cells in one bead. Beads were desiccated with silica gel then directly immersed in liquid nitrogen. Cell viability after thawing was evaluated by its chlorophyll content. The main factors influencing cell viability, such as dehydration rate, water content of beads, recovery methods after thawing, were studied. The results were as follows. Effect of dehydration rate: Various dehydration rates were attained by regulating the weight of silica gel in Petri dishes. At different dehydration rates, all the three algae obtained highest viabilities at the dehydration rate of -0.9% water content/h. Cell viability was low at either high or low dehydration rate. This is perhaps related to their own tolerance against desiccation and freezing. Some reports showed that several green algae also achieved high viability at the dehydration rate of -0.9% water content/h using encapsulation-dehydration. It is possible that the dehydration rate of -0.9% water content/h is common to many marine microalgae. Effect of water content on cell viability: Water content of plant material is the key factor influencing cell viability using encapsulation-dehydration. For every alga species, the optimum water content of beads before being freezen is: P. viridis 35%, I. zhanjiangensis and I. galbana 3011, 30%. The highest viability of P. viridis reached 74.4% when it was dehydrated to its optimum water content. As for other two algae, their highest viabilities were 15.3% and 17.4% respectively. Some research on green algae reported 40% optimum water content. This research, however, showed the optimum range of water content for the three golden-brown algae is between 30% and 35%.
出处
《海洋与湖沼》
CAS
CSCD
北大核心
2005年第2期172-178,共7页
Oceanologia Et Limnologia Sinica
基金
国家自然科学基金资助项目
30 170 0 99号
30 4 70 184号
辽宁省教育厅基金资助项目
2 0 0 4 10 15号
中国科学院知识创新工程资助项目
ZKCX2 2 11号
