摘要
研究了包埋干燥法超低温保存‘品丽珠’葡萄离体茎尖的影响因素———芽的位置、干燥时间、预冰冻温度及材料生理状态。选继代培养 4个月以上的侧芽 ,蔗糖预培养3d、干燥 5h ,两步降温和直接在MS培养基上培养 ,存活率达 4 0 %。
Bud location,dehydration,prefreezing and subculture time were studied on grapevine Cabernet France shoot tip cryopreservation by the encapsulation dehydration process.Best result(40%)was obtained in the following procedare:70 select lateral buds after four months’subculture as explants,preculture them for 3 days,dehdrate for five hours then cool them in two steps and finally subculture them on standard culture medium.
出处
《园艺学报》
CAS
CSCD
北大核心
2001年第1期62-64,共3页
Acta Horticulturae Sinica
基金
河北省自然科学基金资助项目! (399485 )
国际植物遗传资源研究所自然科学基金资助项目
