摘要
目的探讨热应激预处理对大鼠肢体缺血再灌注损伤的影响。方法制备大鼠缺血再灌注模型及热应激预处理模型。采用硫代巴比妥酸法测定大鼠丙二醛(MDA)浓度,采用黄嘌呤氧化酶法测定超氧化物歧化酶(SOD)活力并与对照组比较。结果大鼠肢体缺血再灌注后血清中的MDA含量明显增高(P<0.01),并有随再灌时间延长不断升高的趋势,而SOD活性明显降低(P<0.01);热应激预处理后,血清中MDA含量明显降低(P<0.01),SOD活性明显增高(P<0.01)。结论热应激预处理可增强SOD清除细胞内氧自由基的水平。
Objective To study the effect of pretreatment with heat stress on ischemia-reperfusion injuries of the limbs in rats. Methods Rat models of ischemia-reperfusion injury were established with or without prior heat stress treatment, in which serum malondialdehyde (MDA) level and superoxide dismutase (SOD) activities were measured by means of thiobarbituric acid and nitroblue tetrazolium respectively. Results Comparison between ischemia-reperfusion group and the control group revealed significantly elevated MDA levels (P<0.01) and depressed SOD activities (P<0.01) in the condition of ischemia-reperfusion injury. Pretreatment with heat stress, however, significantly lowered the elevated MDA levels (P<0.01) and enhanced SOD activities (P<0.01) during ischemia-reperfusion injury. Conclusion Pretreatment with heat stress can enhance the activity of SOD to remove free oxygen radicals in the cells.
出处
《第一军医大学学报》
CSCD
北大核心
2002年第6期506-508,共3页
Journal of First Military Medical University
基金
九五卫生医药基金(98D36)
