摘要
采用聚丙烯酰胺凝胶电泳分析四川茶亚属(Thea Chang)2个种的14个样本叶片的过氧化物酶同工酶。结果表明,茶树过氧化物酶同工酶可分为两大酶区。第1酶区相对迁移率为0.39～0.53;第Ⅱ酶区为0.59～0.75。第Ⅱ酶区的6、7、8条酶带宽而色深,出现频率均在50%以上;3、5、8条酶带在所有的样本中都存在。按排序结果分为4个类型。云南大叶茶与四川盆地南沿山地茶树亲缘关系较近,表明它们可能有相同的起源,川南盆沿也是茶树原产地之一。
Analysis of 14 samples of two tea species(Camellia sinensis and C.gymnogyna)in Sichuanfor their peroxidase isozymes by polyacrylamide gel plate electrophoresis showed that their zymo-grams could be divided into two distinct regions,with the Rf value ranging from 0.39 to 0.53 and0.59 to 0.75,respectively.The 6th,7th and 8th bands in the second region were broad in widthand deep in colour,the frequencies of their appearance being above 50%.The 3rd,5th and 8thbands were common to all the samples investigated.The sequencing method was adopted to studythe similarities of the samples in their zymograms and,as a result,they were divided into 4 types.The Yunnan big lesf tea(C.sinensis)appeared to be closely related to C.gymnogyna,which growsin the mountainous regions adjacent to the south Sichuan Basin.They might share a common an-cestor.It is presumed that the mountainous regions in South Sichuan might be part of the nativeplace of Camellia.
出处
《西南农业大学学报（自然科学版）》
CSCD
1991年第3期338-343,共6页
Journal of Southwest Agricultural University
基金
国家自然科学基金
关键词
茶树
过氧化物酶
同功酶
亲缘关系
Camellia
peroxidase isozyme
genetic relation
Sichuan province
