摘要
以樱桃番茄（Ｌ．Ｅｓｃｕｌｕｎｔｕｍ ｖ．Ｃｅｒａｓｉｆｏｒｍｅ）的下胚轴、幼叶、茎尖及带芽茎段为外植体，在附加不同浓度ＢＡ和ＩＡＡ的ＭＳ培养基上进行离体培养，结果表明：樱桃番茄“红宝石”品种的下胚轴、叶和茎尖的最佳芽诱导培养基分别为ＭＳ＋２．０ｍｇ／ＬＢＡ＋０．５ｍｇ／ＬＩＡＡ；ＭＳ＋２．０ｍｇ／ＬＢＡ＋０２ｍｇ／ＬＩＡＡ；ＭＳ＋１．０－２．０ｍｇ／ＬＢＡ＋０．１－０．５ｍｇ／ＬＩＡＡ芽最佳增殖培养基 ＭＳ＋１．０ｍｇ／Ｌ ＢＡ＋０．５ｍｇ／ＬＩＡＡ，其增殖系数为３－４．用带侧芽的无菌短枝进行离体扦插，可１次成苗，扦插培养基为ＭＳ＋０．００５－０．０１０ｍｇ／ＬＮＡＡ或不加激素。
Four kinds of tissue of Lycopersicon Esculentum var. Cerasiforme, hypocotyl, leaf, shoot-tip, and stem with a lateral bud cut from cultivars 'Hong Bao-shi' are cultured on MS medium with BA and JAA at different levels. The results show that the optimal induction media of buds are MS + 1 .0- 2.0 mg/L BA + 0.5 mg/L IAA, MS + 2.0 mg/L BA+0.2 mg/L LAA, MS + 1.0.- 2.0 mg/L BA + 0.1 -.0.5 mg/LIAA. Proliferation medium of shoots is MS + 1.0 mg/L BA + 0.5 mg/L IAA. Induction of mot is better for shoots in MS + 0. 1 mg/L NAA. The cuttage of the stem in vitro are carried on MS + 0.005 -0.01 mg/L NAA or MS without hormone. The rate of proliferation of the shoots is 5.
出处
《西北师范大学学报（自然科学版）》
CAS
2002年第2期64-67,共4页
Journal of Northwest Normal University(Natural Science)
