摘要
为了探索油菜外植体分化再生的机理,利用扫描电镜技术对芥菜型油菜DB3的下胚轴、子叶在不同的离体培养时期的细胞分化和形态变化进行了研究。结果表明,下胚轴外植体是切面上的维管束薄壁细胞和距离切面400～600μm的薄壁细胞脱分化,然后再分化形成芽。而子叶仅仅是距离切面400～600μm的薄壁细胞脱分化后再分化成芽的。
In this study,hypocotyl and cotyledon of B.juncea,cultured in vitro at different time,were scanned by scanning microscope.The results indicated there were two kinds of cells de-differentiated and then differentiated shoots in hypocotyls. One was parenchyma cells of hypocotyl vascular bundle on cut face,the other was parenchyma cells about 400-600 μm away from cut face. But parenchyma cells of cotyledon did only from the later.
出处
《华北农学报》
CSCD
北大核心
2005年第2期23-25,共3页
Acta Agriculturae Boreali-Sinica
基金
河南省自然科学基金项目(0124160310)
