摘要
建立了高效液相色谱法准确、高效测定油脂混合体系中β-谷甾醇含量的方法，并对油脂混合物中β-谷甾醇的提取工艺进行了优化。采用C18反相色谱柱对β-谷甾醇含量进行测定，流动相为甲醇-水（体积比98．5：1．5），检测波长210 nm。结果表明：β-谷甾醇在25~500μg/mL范围内，质量浓度与峰面积呈良好的线性关系（R2=0．9998），仪器检出限为7．5μg/mL，相对标准偏差（RSD）为0．85%；油脂混合物中β-谷甾醇的最佳提取工艺条件为提取次数4次，离心转速2000 r/min，皂化温度80℃，皂化时间210 min；在最佳提取工艺条件下β-谷甾醇的回收率为93．44%~96．48%，RSD为1．70%。
The accurate and efficient method for determing β-sitosterol content of oil mixture by HPLC was established,and the extraction process of β-sitosterol from oil mixture was optimized. C18 reverse-phase chromatography column was used for the determination ofβ-sitosterol content under the conditions of mobile phase methanol - water （volume ratio 98. 5：1. 5 ） and detection wavelength 210 nm. The re-sults showed that the mass concentration of β-sitosterol had a good linear relationship with peak area in the range of 25-500μg/mL. The correlation coefficient, the instrumental limit of detection and the rela-tive standard deviation （RSD） were 0. 999 8, 7. 5 μg/mL and 0. 85% respectively. The optimal extrac-tion conditions of β-sitosterol from oil mixture were obtained as follows：extraction times four, centrifugal speed 2 000 r/min, saponification temperature 80℃ and saponification time 210 min. Under these conditions,the recovery rate and RSD of β-sitosterol were 93. 44% -96. 48% and 1. 70%, respectively.
出处
《中国油脂》
CAS
CSCD
北大核心
2014年第7期91-95,共5页
China Oils and Fats
