摘要
目的:建立超声波辅助萃取-高效液相色谱法快速检测花生中β-谷甾醇的分析方法,并应用该方法对辽宁、山东和河南3个花生种植大省不同品种花生中β-谷甾醇的含量进行分析测定。方法:样品经85%乙醇溶液(乙醇∶水=85∶15,V∶V)超声提取,离心,中性氧化铝去色素和杂质后,经Agilient ZORBAX SB-C_(18)色谱柱分离,二极管阵列检测器检测,外标法定量。结果:β-谷甾醇在1.0~100.0 mg·L^(-1)范围内与峰面积线性关系良好,回归方程为y=10.070x+2.348(r=0.9999);平均加标回收率为88.5%~106.9%,相对标准偏差为2.31%~4.73%(n=6),检出限为0.5 mg/100 g。运用该方法对30个花生样品进行检测,不同品种花生β-谷甾醇含量在31.6~77.0 mg/100 g。结论:该方法简单快捷、重复性好、准确度和灵敏度较高,适用于花生中β-谷甾醇的快速检测。
Objective:To establish a rapid analytical method for the determination ofβ-sitosterol in peanuts by ultrasonic-assisted extraction-high performance liquid chromatography(HPLC).The method was applied to determine the contents ofβ-sitosterol in different peanut varieties from Liaoning,Shandong and Henan province.Method:The samples were extracted with 85%ethanol(ethanol∶water=85∶15,V∶V)by ultrasonic,after centrifugation and removing pigments and impurities with neutral alumina,separated by Agilient ZORBAX SB-C_(18) column,and then detected by diode array detector,and quantitated by external standard method.Result:β-sitosterol showed a good linear relationship with peak area in the range of 1.0~100.0 mg·L^(-1),and the regression equation was y=10.070x+2.348(r=0.9999).The average recoveries were 88.5%~106.9%,with RSD of 2.31%~4.73%,and the limit of detection was 0.5 mg/100 g.The method was used to detect 30 peanut samples,the content of the content ofβ-sitosterol in different peanuts ranged from 31.6 mg/100 g to 77.0 mg/100 g.Conclusion:The method has the advantages of simple,convenient,good repeatability,high accuracy and sensitivity,which can meet the requirements of rapid analysis ofβ-sitosterol in peanuts.
作者
闫超杰
章程
张旭东
王诗琦
孙晓东
YAN Chaojie;ZHANG Cheng;ZHANG Xudong;WANG Shiqi;SUN Xiaodong(Comprehensive Technical Service Center of Jinzhou Customs,Jinzhou 121013,China;College of Environment and Bioresources,Dalian Minzu University,Dalian 116600,China)
出处
《食品安全导刊》
2024年第1期104-107,111,共5页
China Food Safety Magazine
基金
辽宁省自然科学基金项目(2022-MS-455)。
