摘要
目的:观察电针对胰岛素抵抗模型大鼠学习记忆能力与下丘脑中PI3K、Akt2及其磷酸化蛋白表达的影响。方法:将60只Wistar大鼠随机分为正常组、模型组、预防组和电针组,以标本配穴法电针治疗。检测各组大鼠FBG、FIN;空间学习记忆能力;下丘脑Aβ1-42阳性表达、PI3K、Akt2及其磷酸化蛋白表达。结果:(1)较正常组大鼠,模型组大鼠FBG、FINS、ISI升高,IRI降低(P<0.01);定位航行与空间探索时间延迟,跨越次数减少(P<0.01);下丘脑Aβ1-42阳性表达减少,PI3K、Akt2及其磷酸化蛋白表达减少(P<0.01)。(2)较模型组大鼠,预防组和电针组大鼠FBG、FIN、IRI降低,ISI升高(P<0.01);定位航行与空间探索时间缩短,跨越平台次数增加(P<0.01);下丘脑Aβ1-42阳性表达增加,PI3K、Akt2及其磷酸化蛋白表达增加(P<0.01)。(3)较电针组大鼠,预防组大鼠FBG、FIN、IRI降低,ISI升高(P<0.01);定位航行时间缩短(P<0.01);下丘脑PI3K、Akt2及其磷酸化蛋白表达增加(P<0.01)。结论:电针改善胰岛素抵抗模型大鼠学习记忆能力的作用机制可能与改善中枢胰岛素抵抗状态相关。
Objective:To observe the electro-acupuncture effect on cognitive function and the hypothalamic PI3 K,Akt and the phosphorylated protein expression levels of insulin resistance model rats.Methods:Sixty Wistar rats were divided into normal group,model group,protecting electro-acupuncture group and electro-acupuncture group randomly,15 rats in each group.Blood glucose levels and FIN were detected before and after treatment.Aβ1-42 expression was detected by ELISA method and protein levels of hypothalamic PI3 K and Akt were detected via western-blotting method.Results:Compared with normal group,FBG,FINS and ISI raised in model group and IRI reduced(P<0.01).The time in spatial navigation and space exploration delayed(P<0.01).Aβ1-42 expression,protein levels of hypothalamic PI3 K,Akt and the phosphorylated protein expression decreased(P<0.01).Compared with model group,FBG,FINS and ISI reduced in P-EA group and EA group and IRI increased(P<0.01) and the time in spatial navigation and space exploration was shortened(P<0.01).Aβ1-42 expression,protein levels of hypothalamic PI3 K,Akt and the phosphorylated protein expression increased(P<0.01).Compared with EA group,FBG,FINS and ISI reduced in P-EA group and IRI increased(P<0.01)and the time in spatial navigation was shortened(P<0.01).The protein levels of hypothalamic PI3 K,Akt and the phosphorylated protein expression decreased(P<0.01).Conclusion:The mechanism of electro-acupuncture improvement on learning and memory ability in insulin resistance model rats,is pertaining to the regulation of electro-acupuncture on hypothalamus insulin sensitivity in model rats.
作者
王静芝
张晓明
柳梅华
吴松
刘建民
周焕娇
王华
WANG Jingzhi;ZHANG Xiaoming;LIU Meihua;WU Song;LIU Jianmin;ZHOU Huanjiao;WANG Hua(College of Acupuncture Moxibusfion and Orthopaedics,Hubei University of Chinese Medicine,Hubei Provincial Collaborative Innovation Center of Preventive Treatment by Acupuncture and Moxihustion,Wuhan 430061,Hubei, China;Wuhan Mechanical Technology School Infirmary,Wuhan 430075,Hubei,China)
出处
《辽宁中医杂志》
CAS
2019年第1期164-168,共5页
Liaoning Journal of Traditional Chinese Medicine
基金
国家自然科学基金青年项目(81704188)
2017湖北省自然科学基金青年项目(2017CFB184)
湖北中医药大学校级课题(XJ2014KJ004)
关键词
电针治疗
胰岛素抵抗
标本配穴
P13K
Akt
electro-acupuncture
insulin resistance
combination of Biao-Ben acupoints
PI3K
Akt
