摘要
采用神经元急性分离和膜片箝技术以及细胞贴附式方式记录通道活动 ,探讨DHP类Ca2 +通道激动剂BayK8644及拮抗剂nifedipine对下丘脑神经元L 型Ca2 +通道的影响。结果显示 ,在BayK8644作用下 ,通道开放形式发生变化 ,明显可见多级开放 ;通道平均开放时间、平均开放概率显著增加 ,但单通道电导无明显变化。nifedipine的作用与BayK8644相反。结果提示 ,BayK8644对下丘脑神经元L 型Ca2 +通道有明显激动作用 ,nifedip
To investigate the effects of Bay K8644 and nifedipine on L type of Ca 2+ channels in hypothalamic neurons, the cell attached patch clamp technique was used to record Ca 2+ current from isolated neurons. The results showed that the averaged open time and NPo were markedly increased by Bay K8644, but had no effect on channel conductance. Nifedipine, on the contrary, had an inhibitory effect on the L type Ca 2+ channels.
出处
《生理学报》
CAS
CSCD
北大核心
2001年第5期339-343,共5页
Acta Physiologica Sinica
基金
ThisresearchwassupportedbytheNationalNaturalScienceFoundationofChina (No 39970 810
3992 90 0 1)
