摘要
目的：建立急性分离大鼠皮层神经元的方法。方法：切取活脑组织切片,经Protease酶解消化、孵育后,采用酶加机械分离法制备10~16 d鼠龄的大鼠皮层神经元。作形态学观察,用全细胞膜片钳技术记录其电生理学特性。结果：分离出的神经元倒置相差显微镜下形态保持良好,有较长的轴突;用膜片钳技术证实,80%以上细胞可以记录出电活动,其保存了主要的离子通道活性。结论：成功建立了一种经济、简便的适用于膜片钳技术的大鼠皮层神经元急性分离方法。
Objective： To establish a method which is described for separating the rat cortical neurons.Methods： Cuting a slice of live brain sections,after Protease enzymatic hydrolysis digestion,incubation,the cortical neurons were obtained by a combination of mechanical and enzymatic means from 10-16 day-old rats.Observing by morphology,their electrophysiological properties were recorded by whole-cell patch-clamp recording technique.Results： the difference of the inverted cortical neurons which were obtained by separation kept good under microscope.They each had a long axon.Proved by the patch-clamp recording technique,more than 80% cells could be recorded their electric activity,which preserved the ion channel activity.Conclusion： An economic and simple method was established,which is described for separating the rat cortical neurons by the patch-clamp recording technique.
出处
《中国医药导报》
CAS
2011年第18期41-43,共3页
China Medical Herald
基金
黑龙江省齐齐哈尔市科技局资助项目(项目名称:AA对大鼠皮层神经元Ik的作用及信号转导途径的研究)
关键词
皮层神经元
急性分离
膜片钳
离子通道
Cortical neurons
Acute isolation
Patch clamp
Ion channel
