摘要
目的建立一种适用于膜片钳技术的大鼠皮层神经元急性分离改良方法,并对其离子通道电流进行记录。方法采用酶加机械分离法制备12～16d鼠龄的大鼠皮层神经元。其电生理学特性测定用全细胞膜片钳技术。结果分离出的神经元形态正常,有较长的轴突;用膜片钳技术证实,其保存了主要的离子通道活性。结论成功建立了一种简单快速的适用于膜片钳技术的大鼠皮层神经元急性分离改良方法。
Objective To develop a modiffied method of acute isolation of rat cortex neurons,and observe its ion current with patch clamp.Methods The cortex neurons were obtained by a combination of mechanical and enzymatic means from 12 16 day old rats.Patch clamp technique was used to measure the electrophysiological properties of the neurons.Results The neurons isolated in this way had regular configuration. The neuraxons of them were very long.With patch clamp techniques,we found that the methods could save the activities of primary iron channels.Conclusion A simple and fast method for acutely isolating rats cortex neurons suitable for patch clamp is successfully found.
出处
《哈尔滨医科大学学报》
CAS
北大核心
2005年第3期285-287,共3页
Journal of Harbin Medical University
基金
黑龙江省卫生厅资助课题(2003097)
关键词
皮层神经元
急性分离
膜片钳
离子通道
cortex neuron
acutely isolating
patch clamp
ionic channel
