摘要
用淋巴细胞杂交瘤技术，制备出５株抗ＨＣＶ－ＮＳ３区单克隆抗体（ＭｃＡｂ），分别命名为Ｃ７－１，３，６，８，９。单抗特性测定结果显示５株单抗均为ＨＣＶ－ＮＳ３区特异性，与ＨＣＶ其它区域的抗原及宿主茵成份均无交叉反应；此５株单抗识别ＮＳ３区上两个不完全相关的抗原位点，其中Ｃ７－１，３，６株识别同一抗原决定簇；Ｃ７－９的识别位点与此３株有一定的相关；Ｃ７－８则识别与此４株不完全相关的位点。ＨＣＶ－ＮＳ３抗体阳性病人血清对３株单抗显示不同程度的竞争抑制作用，揭示自然抗原上存在这两个不完全相关的抗原位点，并为单抗进一步分析ＨＣＶ自然抗原及感染提供了理论基础。
Five
hybridomas, named C7-1,3,6,8 ,and 9 were established by hybridoma
technique.Characterization of these hybridomas demonstrated that
these five monoclonal antibodies(McAbs)were all specific for HCV-NS3
antigen and had no cross-reactivity with any other anti-gens of HCV
and E. coli components. They recognized two incomplete-related
epitopes on NS3antigen. HCV-NS3 antibody positive sera inhibited the
binding of McAbs to antigen with differ-ent extent.These results
indicated that there were two epitopes on natural antigen of HCV
asthe same as th on engineering antigens. It also shows that McAb to
HCV is useful to analysis ofHCV antigens and study of HCV infection.
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
1995年第5期278-279,289,共3页
Chinese Journal of Immunology
