摘要
运用高压均质法制备了加载尼美舒利的纳米结构脂质载体(Nanostructured Lipid Nanoparticles,NLC),采用单因素设计考察了固液态油脂比、脂质用量、乳化剂用量、药脂比、均质压力和均质次数对纳米粒质量的影响。采用正交设计优化了制备条件。确定的最适宜制备方案为油酸与单硬脂酸甘油酯(glycerin monostearate,GMS)投料比为3∶10,药脂比为1∶40,TPGS用量为1.4 g,均质压力为90 MPa。依据最适宜处方制备的纳米粒粒径为132.6±1.4 nm,包封率为82.35±1.23%。扫描电镜显示所制备纳米粒呈类球形,体外释放实验表明其释放行为符合Higuchi模型。
High pressure homogenization method was used to prepare Nimesulide loaded nanostructured lipid nanoparticles(NLC).Single factor design was chosen to study the influence of solid-liquid lipid ratio,amount of lipid,amount of emulsifier,drug-lipid ratio,homogenization pressure and homogenization cycles on quality of the prepared nanoparticles.Orthogonal design was used to optimize the preparation parameters.The optimal preparation method was 3∶ 10 of oleic acid-glycerin monostearate(GMS) ratio,1∶ 40 of drug-lipid ratio,1.4 g of TPGS,90 MPa of homogenization pressure.Particle size and entrapment efficiency of NLC prepared by optimal conditions were 132.6 ± 1.4 nm and 82.35 ± 1.23%,respectively.The scanning electron microscope showed that the nanoparticles were of spherical shape.The in vitro release study showed that release behavior accorded with Higuchi model.
出处
《化学工业与工程》
CAS
2014年第4期46-51,共6页
Chemical Industry and Engineering
关键词
正交设计
NLC
高压均质法
尼美舒利
优化
orthogonal design
nanostructured lipid nanoparticles(NLC)
homogenization method
Nimesulide
optimization