摘要
目的通过分析2012年重庆三峡库区蚊虫中分离乙脑病毒株PrM及E基因序列，揭示毒株的遗传特性。方法对新分离乙脑病毒的PrM及E基因进行PCR扩增，将扩增产物测序。用分子生物学软件进行核苷酸及氨基酸序列分析，并绘制系统进化树。结果新分离的8株乙脑病毒株均为基因I型，与我国其他省市的既往分离株进化关系较近；分离株与减毒活疫苗株SA14—14—2相比较，PrM区核苷酸同源性在85．1％～86．7％之间，氨基酸同源性在95．1％～96．3％之间；E基因区核苷酸同源性在87．6％～87．8％之间，氨基酸同源性在96．9％～97．1％之间。所有新分离株与疫苗株在E基因存在14个氨基酸差异位点。结论2012年8株重庆乙脑分离株为基因I型，在E基因区域与疫苗株相比有部分差异。
Although the incidence rates of Japanese encephalitis （JE） were relatively high in Chongqing, southwest China, genetic characterization of indigenous Japanese encephalitis viruses （JEV） strains remained incomplete. In this study, partial prM and full length envelope genes from 8 mosquito JEV isolates in 2012 from the Three Gorges District in Chongqing were amplified. Phylogenetic analysis indicated that these 8 strains were classified to Genotype I, closely related to isolates from other provinces in China from 1979 to 2005. Compared with the vaccine strain SA14-14-2, homology in nucleotide and amino acid sequence for partial prM gene were 85. 1%-86. 7% and 95. 1%-96.3%, respectively, while those for the envelope gene were 87.6 % -87.8 % and 96.9 %-97.1 %, respectively. Although 14 variations in amino acid residues were observed in the envelope protein between SA 14-14-2 and new isolates, none of them associated with neurovirulence of JEV. These results, as etiological data for JE-related vector surveillance in Chongqing, will benefit local control and prevention of JE in the future.
出处
《中国人兽共患病学报》
CAS
CSCD
北大核心
2014年第6期622-627,共6页
Chinese Journal of Zoonoses
关键词
乙脑病毒
序列分析
E基因
PrM基因
Japanese encephalitis virus （JEV）
sequence analysis
envelope gene
PrM gene
