摘要
目的建立检测猪血清中流行性乙型脑炎病毒(JEV)IgG抗体的间接免疫荧光试验(IFA)。方法用乙脑病毒感染C6/36白蚊伊蚊细胞制备抗原片,建立检测猪血清中JEV-IgG抗体的IFA方法,并用于猪乙脑血清流行病学调查。结果成功建立特异的猪JEV-IgG的IFA检测方法,并对50份母猪、55份仔猪和65份屠宰猪的血清进行检测。乙脑血清抗体效价≥1:200的分别为88.00%,10.91%和24.62%。结论建立的IFA方法能较好的用于猪血清乙脑流行病学调查。
To establish an indirect immunofluorescence assay （IFA） to detect IgG antibody to Japanese encephalitis virus （JEV）in sera of pigs, the C6/36 cells infected with JEV were used to prepare the cell antigen smears, so as to establish a method of assay to detect the IgG antibody to JEV in sera of pigs as well as to be used in the epidemiological surveys. It was found the positive detection rate of anti JEV-IgG antibodies with antibody titers of more than 1 ： 200 in serum specimens from 50 cases of sows, 55cases of young pigs and 65 cases of hogs were 88.00%, 10.91% and 24.62% respectively. It is evident that this specific IFA assay can be used for the epidemiological investigations for the status of JEV infection in pigs.
出处
《中国人兽共患病学报》
CAS
CSCD
北大核心
2006年第2期150-152,共3页
Chinese Journal of Zoonoses
关键词
乙型脑炎病毒
间接免疫荧光
IGG抗体
Japanese encephalitis virus
indirect immunofluorescnce assay
IgG antibody
