摘要
针对目前南瓜杂交种纯度SSR鉴定方法操作繁琐,难以用于生产实践的问题,笔者介绍了一种简便的南瓜杂交种纯度SSR检测方法。该方法的基因组提取过程不需研磨、冷冻、离心、晾干等操作步骤,检测周期小于3 min,PCR反应体系与聚丙烯酰胺凝胶电泳的银染步骤,比常规方法更节约时间和成本,整个检测周期小于4 h,每人每天可完成480个样品的检测,且结果与田间鉴定结果吻合率达到99%。该方法可为南瓜杂交种的大规模快速纯度鉴定提供依据。
Because of the existing Cucurbita moschata hybrid purity identification system based on SSR was complicated and difficult for the production of practice, a simple Cucurbita moschata hybrid purity identification system based on SSR was introduced here. Without grinding, freezing, centrifugation and drying, the whole genomic DNA extraction period was less than 3 minutes. Comparing to the conventional method, the PCR detection system and the silver stained steps saved time and cost, the whole process was less than 4 hours, one person a day can complete 480 hybrids detection easily, and the coincidence rate of the simple SSR-based purity with the field test results was 99%. This method would provide the base for large-scale rapid purity identification of Cucurbita moschata hybrids.
出处
《中国农学通报》
CSCD
2014年第7期194-198,共5页
Chinese Agricultural Science Bulletin
基金
农业部公益性行业(农业)科研专项"南瓜产业技术研究与示范"(201303112)
"十二五"农村领域国家科技计划"南瓜杂种优势利用与新品种选育"(2012BAD02B03-17)
关键词
南瓜
杂交种
SSR
纯度鉴定
Cucurbita moschata
hybrids
SSR
purity identification
