摘要
目的 运用PrimerPremier 5 0软件设计PCR引物 ,并且检验所设计引物的PCR扩增效率和特异性。方法 运用PrimerPremier 5 0软件设计 16对猪和犬的PCR扩增引物 ,通过PCR扩增后进行琼脂糖凝胶电泳检测实验结果。结果 在所设计的 16对引物中有 8对PCR扩增特异性好且效率高 ,成功率 5 0 %。但是 ,其中早期设计引物 12对只有 4对成功 ,后期设计引物 4对全部成功。结论 从引物设计的过程中可以看到一种趋势 -后期引物设计的成功率远远高于早期。这表明我们在引物设计方面正在逐步成熟 。
Objective With the help of Primer Premier 5.0 to design PCR primer and verifying the efficiency and speciality of PCR about these primers. Methods Sixteen pairs of primers that would be amplified in Genomics of pig and dog were designed by Primer Premier 5.0. The results of PCR would be investigated through agarose gel electrophoresis.Results Eight pairs of primers in all designed succeeded in the efficiency and speciality of PCR, the ratio of success was 50 percent. The twelve pairs of primers what were designed in the forepart of the experiment were only four to amplify better. However, the four pairs of primers designed in the anaphase all succeeded. Conclusions There is a trend that can be recognized in the process of designing primer. Namely, the forepart is far more than the anaphase in the ratio of success to design primer. It is indicated that our technology to design primer is further professional. Moreover, the most important is that the technique route of using the comparative genomics to isolate new gene is farther comprehended and perfected and placing a direction at next experiment.
出处
《锦州医学院学报》
2004年第6期43-46,共4页
Journal of Jinzhou Medical College
基金
辽宁省科技厅博士启动基金 (2 0 0 2 10 72 )
