摘要
目的研究加味楂曲饮(JWZQY)干预非酒精性脂肪肝的药效学及部分作用机制。方法选用SDF级大鼠,给予高脂饲料建立非酒精性脂肪肝模型,用低、中、高剂量JWZQY进行干预,连续灌胃8周,观察肝脏组织形态学改变,检测肝组织细胞色素氧化酶P4502E1(CYP2E1)mRNA及蛋白表达水平。结果与空白组相比,模型组呈现明显脂肪变性。高、中剂量JWZQY治疗后肝脏脂肪变程度明显减轻,中剂量组CYP2E1mRNA水平明显降低、CYP2E1蛋白表达丰度明显降低。结论 JWZQY可有效防治实验性非酒精性脂肪肝,并降低模型大鼠肝组织CYP2E1 mRNA及蛋白表达水平。
Objective To study the pharmaeodynamics and its part action mechanism of JiaWei ZhaQuYin（JWZQY） intervening non - alcoholic fatty liver. Methods The study selected SDF level rats. The nonalcoholic fatty liver rat models were induced by ahigh fat diet and intervened by JWZQY for 8 weeks. The conventional oil red O method （ modified Lillie） was used to observe the morphological changes of the liver tissue. Quantitative PCR was used to detect liver tissue CYP2E1 mRNA level. Westernlaw Blo- ting was used to detect liver tissue CYP2E1 protein levels. Results The high -fat diet led to liver ceils diffuse enlargement and round in rat the hepatic lobule, the sinusoidal smaller even not visible, the cytoplasm contains a large number of red dye fat drop- lets of varying sizes, showing steatosis. High - dose and medium dose JWZQY can significantly reduce hepatic steatosis. Com- pared with the model group, CYP2ElmRNA levels in middle dose group were significantly lower, CYP2E1 protein abundance in the middle dose group was significantly lower. Conclusion JWZQY can effectively intervene in the experimental non - alcoholic fatty liver,also can reduce CYP2E1 protein and mRNA expression, suggesting that this maybe related to oxidative stress and lipid peroxide.
出处
《时珍国医国药》
CAS
CSCD
北大核心
2014年第4期789-792,共4页
Lishizhen Medicine and Materia Medica Research
基金
国家自然科学基金(No.81202624
81102720
81072902)
成都中医药大学科技发展基金
