摘要
目的考察穿心莲内酯提高产生物被膜（BF）铜绿假单胞菌对左氧氟沙星的敏感性。方法采用琼脂倍比稀释法体外药物敏感性实验，考察穿心莲内酯与左氧氟沙星不同配比的22个样品对160株临床上近期分离的BF阳性铜绿假单胞菌的MIC值，并采用概率单位分析法，评价不同配比受试药物的抗菌活性；构建体外BF的渗透模型，观察穿心莲内酯在300、30、3μg·mL-1剂量下对BF渗透性的影响。结果左氧氟沙星配比穿心莲内酯在1：0．001～1：0．01时，药物的Emax和ED50虽然有一定的波动，但总体趋势上表现出Emax高于和ED50低于单用左氧氟沙星；穿心莲内酯在受试剂量下均可以提高铜绿假单胞菌BF模型对左氧氟沙星的渗透性，在模型构建后的2、4、6、8、10、24h时药物的渗透量显著升高。结论穿心莲内酯在一定剂量范围内有提高左氧氟沙星对产BF铜绿假单胞菌抗菌活性的作用，并且这一作用与药物提高BF的渗透性有关。
OBJECTIVE Study of andrographolide enhancing the sensitiveness of Levofloxacin on Pseudomonas aeruginosa producing biofilm （BF）. METHODS Using experiments recommended and agar dilution method about the drug sensitivity in vitro, we investi- gate the influence of 22 samples of different andrographolide/Levatloxacin ratio on MIC of 160 BF - positive Pseudomonas aeruginosa after clinical separation using probit analysis ; In vitro BF infiltration model was established. Effeetes of 300,30,3 μg. mL - 1 androgra- pholide on the permeability of BF. RESULTS In vitro drug sensitivity resuhs showed that when the ration of Levofloxacin thtoan andrographolide was in 1 ： 0. 001 -1 ： 0.01 ,there were certain fluctuations in E and ED50 ,but the overall trend showed a higher E and lower ED50 than Levofloxaein used alone; Andrographolide could improve the permeability of Pseudomonas aeruginosa＇ s BF, the peruleability of Levofloxacin significantly increased in 2, 4, 6, 8, 10, 24 h after infiltration. CONCLUSION The results of drugin vitro sensitivity experiments suggest andrographolide can improve the antibacterial activity of Levofloxacin at dose within a certain related, which was related to increasing the permeability of Levofloxacin.
出处
《华西药学杂志》
CAS
CSCD
北大核心
2014年第2期128-131,共4页
West China Journal of Pharmaceutical Sciences
基金
成都中医药大学自然科学基金面上项目(批准号:ZRMS201238)
