摘要
目的：应用蒽酮-硫酸法测定维地红涂剂中多糖的含量。方法以无水葡糖糖为对照品，0.2％蒽酮-硫酸试液为显色剂，检测波长620nm。结果多糖在1.9～34.2μg· mL -1范围内线性关系良好，y＝0.033X-0.0067（r＝0.9999），回收率96.0％，RSD＝1.96％（n＝6）。结论该方法用于维地红涂剂中多糖的含量测定方法稳定，结果重现性好。
OBJECTIVE To detemine the content of polysaccharide in Verdihong paint by the anthrone sulfuric acid method.METHODS Using the anhydrous dextrose as the control ,0.2%Anthrone-Sulfuric acid as the stain , The content of Polysaccharide was determined by ultraviolet spectrophotometry at the wavelength of 620 nm.RESULTS The polysaccharide curve was linear within a range of 1.9-34.2μg · mL-1 , linear equation was y =0.033 x -0.0067 （ r =0.9999 ） , the average recovery was 96.0% with RSD of 1.96%（ n =6 ）.CONCLUSION This method is accurate and the results has fine repetition.
出处
《海峡药学》
2014年第3期54-55,共2页
Strait Pharmaceutical Journal
基金
湖北省重大科学技术鉴定成果(登记号:EK2012D 150018000359)
太和科研基金资助项目(2009D34)
关键词
维地红涂剂
多糖
蒽酮-硫酸试液
含量测定
Verdihong paint
Polysaccharide
Anthrone-sulfuric acid method
Content determination
