摘要
目的:提取分离生、熟三七中所含的多糖,并测定其含量;比较生、熟三七中多糖含量的差异性。方法:通过水煮醇沉的方法提取;Sevag法除蛋白;蒽酮-硫酸比色法测定其多糖的含量。结果:蒽酮-硫酸法测定生三七中多糖的含量为6.60%,熟三七中多糖的含量为6.95%。结论:熟三七中的多糖含量高于生三七中的多糖含量。
Objective： To extract,separate and determine polysaccharide in raw and prepared Panax notoginseng,and to find their differences.Methods： The contents of polysaccharide were determined by water extraction and alcohol precipitation methods,Sevag protein-eliminated method,and Anthrone-sulfuric acid colorimetry.Results： The contents of polysaccharide were 6.60% and 6.95% in raw and prepared Panax notoginseng respectively.Conclusion： The contents of polysaccharide in prepared Panax notoginseng is 0.35% higher than it in raw Panax notoginseng.
出处
《河南大学学报（医学版）》
CAS
2010年第4期235-236,共2页
Journal of Henan University:Medical Science
基金
河南大学校内基金项目(B2007050)
关键词
生、熟三七
多糖
蒽酮-硫酸法
raw
cooked panax notoginseng
polysaccharide
anthrone-sulfuric acid colorimetry
