摘要
用蒽酮-硫酸法测定多糖含量的方法被各种测糖的专著收载,而文献报道的酵母多糖含量的测定一般采用的都是苯酚显色法。作者旨在采用蒽酮-硫酸法测定酵母制品多糖的含量,为酵母多糖含量的测定提供一种新方法。结果,摸索出一套适合酵母多糖含量测定的蒽酮-硫酸比色法,采用本方法对酵母培养液进行测定,得到了准确的多糖含量,且本方法具有简单、易掌握、快速等优点,对生产上检测多糖含量具有实用价值。
Determination of polysaccharide by anthranone-sulfuric acid has been recorded by various kinds of books, but it has not been used to estimate the yeast polysaccharide. This text was being to estimate the yeast polysaccharide by anthranonesulfuric acid, and to grope a new way for the determination of yeast polysaccharide. Exact content of yeast polysaccharide has been gained by anthranonesulfuric acid. The anthranonesulfuric acid using to determinate the yeast polysaccharide is a way that is a simple,real time operation and that is easy to palmar grasp. And it has utility value for determination of polusaccharide in produce.
出处
《中国畜牧兽医》
CAS
2007年第1期39-41,共3页
China Animal Husbandry & Veterinary Medicine
基金
哈尔滨市攻关项目(2004AA6BNO20)
关键词
酵母
多糖
蒽酮-硫酸
分光光度法
yeast
polysaccharide
anthranone-sulfuric acid
spectrophotography
