摘要
为比较水提法、碱提法、盐提法及醇提法对家蝇幼虫体外抗炎活性的影响,以脂多糖诱导的巨噬细胞为研究对象,结合不同方法所获产物的产率、蛋白含量等,探明4种方法所获提取物对细胞形态及TNFα、MCP-1分泌的影响。结果表明,碱提法所获产物的产率最高,其次是水提法、盐提法和醇提法;水提法所获产物的蛋白含量最高,其次是盐提法、碱提法和醇提法;水提法所获产物的体外抗炎活性最优,其次是醇提法、盐提法和碱提法。
In order to compare the anti-inflammatory activity effects of the extraction products of housefly maggot obtained by the extraction methods of water, alkali, salt and alcohol, the macrophages which induced by LPS were used in this experiment. The yields and protein content was tested and the effects on the cell morphology and secretion of TNF or, MCP-1 were detected as well. The results showed that the yield of the alkali solution extraction was higher than other methods, followed by the water extraction, salt solution extraction and alcohol extraction. However, the protein content of water extraction was the highest, and the salt solution extraction, alkali solution extraction and alcohol extraction followed. The results also showed that the product of water extraction had the function of anti-inflammatory and was more significant than other methods, the alcohol extraction, salt solution extraction and alkali solution extraction followed.
出处
《广东农业科学》
CAS
CSCD
北大核心
2014年第4期118-121,共4页
Guangdong Agricultural Sciences
基金
国家自然科学基金(81274061)
广东省战略性新兴产业核心技术攻关项目(2012A080800016)
广东省医学科研基金(B2013189)
关键词
家蝇幼虫
抗炎
提取方法
housefly （Musca Domestica） maggot
anti-inflammatory
extraction method
