摘要
目的研究亚慢性铅镉联合染毒对SD大鼠血液生理生化的影响。方法将80只健康SD大鼠,随机分成3个试验组和1个对照组,每日对各试验组大鼠经口给予29.96、89.88和269.65 mg/kg铅镉混合溶液,对照组自由饮水,持续灌胃90 d。每30 d采集一次血液,测定血液生理生化指标。结果与对照组相比,各试验组大鼠血液白细胞总数、红细胞总数和血红蛋白含量总体呈现先上升后下降的显著变化;谷草转氨酶活性、谷丙转氨酶活性和尿素含量持续显著上升;血糖和总胆固醇含量最高剂量组显著下降,甘油三酯含量表现为最低剂量组显著上升;总蛋白、白蛋白、球蛋白、肌酐含量无显著变化。同时,各剂量组大鼠肝细胞和肾小管上皮细胞出现不同程度的颗粒变性、水泡变性和细胞坏死。结论亚慢性铅镉联合暴露对大鼠的血液生理生化指标有显著影响,长期低剂量铅镉联合染毒后可引起动物机体不同程度的肝功能和肾功能损伤。
Objective The combined subchronic effects of exposure to lead acetate [Pb （ NO3 ）2 ] and cadmium chloride [ CdC12 ~ 2.5H20 ] on blood physiological and biochemical indexes of rats were detected to investigate the hazards of environmentally relevant, low-dose exposure to these compounds. Methods 80 SD rats were randomly divided into three experiment groups and one control group. The rats in the experiment groups were orally administrated with Pb（ NO3 ）2 and CdC12 2.5H20 com solution at the doses of 29.96, 89.88 and 269.65 mg/kg for 90 days respectively the rats in control group were orally administrated with water. Blood were collected three bined , and every 30 days to determine physiological and biochemical indexes. Results In each poisoning groups, WBC, RBC and HGB increased during early experiment period and then decreased. ALT, AST and BU increased all the experiment time. GLU decreased in the experiment time. Compared with control group, TC increase at high-dose poisoning group and TG decrease at low-dose poisoning group. The TP, ALB, GLO and CRE in the poisoning groups were not significantly different from those in the control group. And the hepatic cells and renal tubule epithelial cells showed granular degeneration, vacuolar degeneration and necrosis in poisoning groups. Conclusion Low-dose Pb-Cd combined exposure could significantly change physiological and biochemical indexes of blood and cause hepatic and renal pathological injury of SD rats.
出处
《卫生研究》
CAS
CSCD
北大核心
2014年第2期259-264,共6页
Journal of Hygiene Research
基金
国家自然科学基金(No.31101860)
四川省青年科技创新研究团队(No.2013TD0015)
关键词
铅
镉
亚慢性毒性
生理生化指标
Pb, Cd, sub-chronic exposure, physiological and biochemical indexes
