摘要
目的:为防治铅中毒提供实验室研究资料,探讨铅染毒时铅对未成年小鼠骨髓造血干/祖细胞和外周血的毒性作用。方法:60只昆明系小鼠随机分成4组,分别以0mg Pb2+/L、164mg Pb2+/L、546mg Pb2+/L、1638mg Pb2+/L浓度的醋酸铅饮用水对小鼠染毒,于染毒的第10天取小鼠股骨骨髓细胞作体外培养,观察各组红系祖细胞成集落数(BFU-E)和粒系祖细胞成集落数(CFU-GM)的变化并进行外周血测定。结果:醋酸铅可明显抑制骨髓BFU-E和CFU-GM的生长,集落数与对照组相比差异显著(P<0·05),且随醋酸铅浓度的增加,骨髓BFU-E抑制作用增强(相关系数为rs=-0·972,P=0·000),而外周血Hb、WBC和PLT与对照组比较差异无显著性(P<0·05)。结论:骨髓造血祖细胞对铅较为敏感,铅染毒小鼠外周血变化不明显时,已引起骨髓BFU-E和CFU-GM的生长障碍。
Objective： To investigate the toxic effect of acetic acid lead on haematopoietic stem/progenitor cells and peripheral blood in the immature mice of lead poisoning. Methods： 60 mice were divided into four groups and poisoned by acetic acid lead （0, 164 mg, 546 mg, 1 638 mg Pb^2＋/L） . BFU - E, CFU - GM, Hb, PLT and WBC were observed after 10 days. Results： The number of BFU - E and CFU - GM in Pb group was significant lower than that in the control group （P 〈 0. 05） . The number of BFU - E was significantly deceased with the increase of Pb concentrations （ rs = - 0. 972, P = 0. 000）, while there was no significant difference in Hb, WBC and PLT （P 〉 0. 05） after exposed to Pb for 10 days. Conclusion： The haematopoietic stem/progenitor cells are more sensitive to lead. The reduction of BFU - E and CFU - GM was observed before that of Hb, WBC and PLT.
出处
《中国妇幼保健》
CAS
北大核心
2006年第4期543-545,共3页
Maternal and Child Health Care of China
基金
天津医科大学人才启动基金项目
