摘要
以源于黄土高原地区的蛇龙珠和霞多丽葡萄果实为研究对象,通过光谱和液相色谱-质谱联用技术(HPLC-MS/MS)对其理化指标、多酚含量、抗氧化活性及非花色苷单体酚含量进行测定和分析。结果表明,红色品种蛇龙珠果实中的总酚、总类黄酮、总黄烷醇和总花色苷均不同程度地高于白色品种霞多丽,尤其是总黄烷醇含量前者是后者的2.7倍;蛇龙珠果实的抗氧化能力也高于霞多丽品种,在DPPH法和CUPRAC法的测定中,蛇龙珠果实的抗氧化能力分别是霞多丽的1.8和2.7倍;在2个品种中分别检测出25和12种非花色苷酚类物质,其中包括6种黄烷醇、13种黄酮醇、5种羟基苯甲酸和1种羟基肉桂酸物质,它们的组成和含量均有所不同,尤其是儿茶素含量前者是后者的2倍。品种因素对葡萄果实中酚类物质尤其是非花色苷单体酚物质的组成和含量造成不同程度的影响。在选用多种方法对葡萄果实的抗氧化活性进行评价时,测定结果在不同方法间存在协同性与差异性。
To understand the phenolic compounds and antioxidant activities of Cabernet Gernischet and Chardonnay berries, and provide some theoretical evidence for wine-grape berries from the Loess Plateau region, the physicochemical index, the phenolic content, antioxidant activities and non-anthocyanin individual phenolic content of two varieties grape berries were analyzed. The results showed that the contents of total phenolic, flavonoids, flavanols and anthocyanins compounds in red Cabernet Gernischet berries were higher than those in white Chardonnay berries in different extent, especially for flavanols, its content in red berries was 2.7 fold of that in white berries. The antioxidant activities of Cabernet Gernischet berries were 1.8 （for DPPH free radical-scavenging capacity） and 2.7 （for cupric reducing antioxidant capacity, namely CUPRAC） fold of those in white berries, respectively. The total 25 and 12 non-anthocyanin individual phenolic compounds from Cabernet Gernischet and Chardonnay berries were identified, these compounds belonged to 4 classes, including 6 flavanols, 13 flavonol, 5 hydroxybenzoic acid and 1 hydroxycinnamic acid, there were some differences in composition and concentration of these compounds, especially for catechin compound, its content in red berries was 2.0 fold of that in white berries. The grape variety factor had significantly effect on composition and concentration of phenolics, but the influence was inconsistent for different individual phenolic compounds. The antioxidant results between DPPH and CUPRAC methods exhibited cooperativity and difference.
出处
《食品与发酵工业》
CAS
CSCD
北大核心
2014年第3期163-168,共6页
Food and Fermentation Industries
基金
渭南职业技术学院重点项目(WZYZ201301)
国家葡萄产业技术体系(CARS-30-zp-09)
