摘要
目的 研究人类端粒酶反转录酶C27多肽（hTERTC27）过表达对鼻咽癌C666-1细胞移植瘤生长的影响。方法 利用稳定转染质粒pcDNA3.1（对照组）与pcDNA3.1-hTERTC27（hTERTC27组）的C666-1细胞株建立裸鼠鼻咽癌皮下移植瘤模型。观察肿瘤生长情况，绘制肿瘤生长曲线，处死后剥离肿瘤，进行HE染色观察组织形态学变化。采用Western blot法观察肿瘤组织中剪切后的Caspase-3、Caspase-9、PARP以及bax和bcl-2的表达。结果 小鼠荷瘤第8天后，hTERTC27组移植瘤的体积明显小于对照组（P＜0.05），肿瘤的重量明显低于对照组（P＜0.05），局部肌肉浸润现象较少。hTERTC27组移植瘤Caspase-3、Caspase-9、PARP、bax的表达量增加（P＜0.01），而bcl-2的表达量降低（P＜0.01）。结论 hTERTC27减缓裸鼠鼻咽癌皮下移植瘤的生长速度并促进其凋亡，可能与其上调bax蛋白的表达和下调bcl-2蛋白的表达有关。
Objective To investigate the effect of human telomerase reverse transcriptase C27 polypeptide （hTERTC27） over-expression on orthotopic implanted tumor growth which induced by nasopharyngeal carcinoma cells C666-1 in vivo. Methods Stably transfected C666-1 cell lines were used to establish subcutaneously transplanted tumor mouse model. The tumor growth was observed and the tumor growth curve was made by measuring the volumes of tumors every day. HE staining was used to observe the change of histomorphology. The expressions of cleaved Caspase-3, Caspase-9, PARP, bax and bcl-2 were detected by Western blot analysis. Results The in vivo mouse model showed that over-expression of hTERTC27 significantly reduced tumor volume and tumor weight（P 〈 0.05）, and decreased the local muscle infiltration. Over-expression of hTERTC27 significantly up-regulated the expressions of cleaved Caspase-3, Caspase-9, PARP and bax （P 〈 0.01）, and down-regulated the expression of bcl-2 （P 〈 0.01）. Conclusion The results indicate that over-expression of hTERTC27 can obviously inhibit the growth of transplanted tumor in nude mice, which is possibly related to the regulation of bax and bcl-2 expression.
出处
《肿瘤研究与临床》
CAS
2014年第2期94-97,共4页
Cancer Research and Clinic
基金
国家自然科学基金（81301318）
广东省自然科学基金（s2011010001223）
深圳市基础研究项目（JCYJ20120613170218654）
深圳大学基础研究科研项目（201147）作者单位：（林桂淼、李慧、林苏霞、王晓梅）,合成生物学工程实验室（林桂淼、王晓梅、林李家宓）
香港中文大学神经外科肿瘤研究所（林李家宓、吴世明）
