摘要
目的探讨血清基质金属蛋白酶9（MMP-9）水平及基因-1562C〉T多态性与维吾尔族缺血性脑卒中（Is）及其各亚组的关系。方法采用病例对照研究，选择维吾尔族急性IS患者284例。按新的急性脑卒中治疗（TOAST）分型分为动脉粥样硬化血栓形成（AT）组91例、小动脉病变（SAD）组150例和心源性脑检塞（CE）组43例；选择同期健康体检者226例为对照组。采用酶联免疫吸附法检测血清MMP-9水平，聚合酶链反应-限制性片段长度多态性技术检测MMP-9基因-1562C〉T的基因型。结果IS组血清MMP-9水平显著高于对照组（0．308±0．033mg／L比0．087±0．011mg／L，t=7．813，P=0．000），且AT组、CE组显著高于SAD组（0．350±0．030mg／L比0．261±O．029mg／L，t=4．156，P=0．001；0．317±0．043mg／L比0．261±0．029mg／L，t=2．877，P=0．031）。多变量Logistic回归分析显示，血清MMP-9水平增高是Is的独立危险因素（OR：1．012，95％CI：1．007—1．016，P〈0．01）。IS组基因型（x2=3．558，P=0．058）和等住基因（x2=3．567，P=0．059）频率与对照组无显著性差异。AT组和对照组之间基因型（x2=5．097，P=0．024）和等位基因（x2=5．439，P=0．02）频率存在显著差异，其中AT组cT＋TT基因型频率显著高于对照组（24．2％比13．7％）。多变量Logistic回归分析显示，MMP-9基因-1562C〉T多态性并非是缺血性脑卒中的独立危险因素（OR：0．821，95％CI：0．622—1．059，P=0．124），但却是AT的独立危险因素（OR：1．768，95％CI：1．178—2．677，P=0．007）。结论维吾尔族Is患者血浆MMP．9水平增高，以AT亚组增高最为显著；MMP-9基因-1562C〉T多态性与维吾尔族人群IS的发生无明显相关性，但可能与AT型脑卒中相关。
Aim To investigate the correlation of matrix metalloproteinase-9 （MMP-9） levels and MMP-9 -1562C 〉 T polymorphism with acute isehemic stroke （IS） and its ＂Trial of Org 10172 in Acute Stroke Treatment＂ （TOAST） subtypes in Uygur nationality. Methods A total of 284 patients with acute IS were enrolled using the meth od of case-control study, based on the standard of new TOAST classification, 91 patients were atherothrombosis （ AT）, 150patients were small artery disease （ SAD ） and 43 patients were cardioembolism （ CE ）. Meanwhiie, 226 age-and sex- matched physically healthy subjects were used as control group. The levels of serum MMP-9 and - 1562C 〉 T gene poly morphism in each group were measured and analyzed by enzyme-linked immunosorbent assay and restriction fragment length polymorphism. Results The serum MMP-9 level in the IS group was sigrfificantly higher than that in the control group （0. 308 ±0. 033 mg/L vs 0. 087 ± 0. 011 mg/L, t = 7. 813, P = 0. 000）. The serum MMP-9 level in the AT group and CE group were significantly higher than those in the groups of SAD （0. 350 ±0. 030 mg/L vs 0. 261 ±0. 029 mg/L, t =4. 156, P=0.001; 0.317±0.043 mg/L vs 0. 261 ±0.029 mg/L, t =2. 877, P =0.031）. Multivariate Logistic regression analy- sis showed that the increased serum MMP-9 level was an independent risk factor for ischemic stroke （ OR： 1. 012, 95% CI： 1.007 -1.016, P〈0.001）. There was no significant difference in the frequencies of genotype （x2 =3.558, P=0.058） and allele （ X2 = 3. 567, P = 0. 059 ） of MMP-9 - 1562C 〉 T between the IS group and the control group. However, there were significant difference in the frequencies of genotype （ X2 = 5. 097, P = 0. 024） and allele （ X2 = 5. 439, P = 0. 02 ） of MMP-9 - 1562C 〉 T between the AT group and the control group. The CT ＋ TT genotype frequency in the AT group was significantly higher than the control group （24. 2% vs 13.7% ）. Multivariate Logistic regression analysis showed that MMP-9-
出处
《中国动脉硬化杂志》
CAS
CSCD
北大核心
2014年第1期55-60,共6页
Chinese Journal of Arteriosclerosis
基金
新疆维吾尔自治区卫生厅青年科技人才专项科研项目(2009Y05)
乌鲁木齐市科学技术计划项目(Y101310009)
