摘要
目的:探讨白细胞介素-8(IL-8)基因-251(A/T)位点单核苷酸多态性(SNP)与冠心病血瘀证遗传易感性的关系。方法:采用病例-对照研究,以聚合酶链反应-限制性片段长度多态性(PCR-RFLP)的方法,分析136例冠心病血瘀证和122例健康对照组IL-8-251A/T SNP分布情况。结果:位点基因型频率分布结果显示,IL-8-251A/T多态性位点各基因型频率和等位基因频率在CHD血瘀证组和对照组相比,差异有统计学意义(P<0.05)。IL-8-251多态的基因型分布频率在对照组人群中符合Hardy-Weinberg平衡(P=0.984),在有家族史的CHD血瘀证组中AT型基因提高其发病风险,其OR(95%CI)为4.350[1.108,17.073],P<0.05,其余未见有影响。结论:IL-8-251A/T SNP AT基因型可增加家族聚集性冠心病血瘀证的易感性。
Objective： To investigate whether the -251A/T polymorphism of the IL-8 promoter was associated with blood stasis syndrome with coronary artery disease. Method： Polymorphisms of IL-8 were typed using polymerase chain reaction -restriction fragment length polymorphisms in 136 patients with blood stasis syndrome with coronary artery disease and 122 healthy controls. Result： There was significant difference in frequencies of allele and genotype in IL-8-251 A/T polymorphisms between patients with blood stasis syndrome with coronary disease and control group （P〈0.05）. The frequencies in the healthy controls was assistant of Hardy-Weinberg balance （P=0.984）. The levels of genotypes AT in blood stasis group CHD patients with family history were significantly higher than control group, OR （95%CI） = 4.350 [1.108, 17.073], P〈0.05. Conclusion： Genotypes ATmight increase susceptibility of familial aggregation of blood stasis syndrome of CHD.
出处
《中华中医药杂志》
CAS
CSCD
北大核心
2015年第9期3286-3289,共4页
China Journal of Traditional Chinese Medicine and Pharmacy
基金
国家自然科学基金青年项目(No.81102595)
广西自然科学基金项目(No.2012GXNSFAA053113)
广西壮族自治区卫生厅中医药科技专项课题(No.GZPT1219)
广西壮族自治区卫生厅科研课题(No.Z2012181)
广西高等学校优秀中青年骨干教师培养工程(No.桂教人[2013]16号)
广西科学研究与技术开发计划项目(No.桂科合1347004-26)~~
关键词
白细胞介素-8
冠心病
血瘀证
基因多态性
Interleukin-8
Coronary heart disease
Blood stasis syndrome
Gene polymorphism
