摘要
目的 探讨血浆微小RNA在早期胃癌中的表达情况,研究其在胃癌发生发展中的意义.方法 本研究首先对30例胃癌患者和30例健康对照人群血浆中的15种微小RNA（miR-1、miR-106a、miR-106b、miR-17-5p、miR-20a、miR-21、miR-221、miR-27a、miR-34、miR-376c、miR-378、miR-423-5p、miR-451、miR-486、miR-744）进行检测,然后通过定量逆转录聚合酶链反应来确定另外60例胃癌患者和60例健康对照人群的血浆微小RNA是否存在差异.通过受试者工作特征曲线（ROC曲线）下面积来检测被筛查出的血浆微小RNA诊断胃癌的敏感性和特异性.结果 胃癌患者的3种血浆微小RNA（miR-106b、miR-20a和miR-221）水平较健康对照人群有显著增高（P＜0.05）.用miR-106b、miR-20a和miR-221检测胃癌的ROC曲线下面积分别为0.773（95％CI0.776～0.841）、0.859（95％CI0.805～ 0.914）和0.796（95％ CI0.726 ～0.866）.胃癌患者的这3种微小RNA在每一个TNM分期中的表达水平与健康对照人群比较差异有统计学意义（P＜0.05）,而在各TNM分期表达水平之间比较差异无统计学意义（P＞0.05）.结论 血浆miR-106b、miR-20a和miR-221可作为胃癌早期检测潜在的生物标志物.
Objective To study the exprssion of miR-106b,miR-20a and miR-221 in early development of gastric cancer.Methods The plasma expression of 15 selected microRNAs （miR-1,miR-106a,miR-106b,miR-17-5p,miR-20a,miR-21,miR-221,miR-27a,miR-34,miR-376c,miR-378,miR-423-5p,miR-451,miR-486,miR-744） in 30 gastric cancer （GC） patients and 30 age-and gendermatched healthy controls were measured and then the differences of those micro-RNAs expressions in other 60 GC patients and 60 matched controls were evaluated by using quantitative reverse transcription polymerase chain reaction.The areas under the receiver operating characteristic （ROC） curves were used to test the sensitivity and specificity of GC diagnosis using these identified plasma microRNAs.Results Three plasma microRNAs miR-106b,miR-20a,and miR-221,were significantly elevated in GC patients than in healthy controls （P 〈 0.05）.Furthermore,the areas under the ROC curves using miR-106b,miR-20a,and miR-221 for GC diagnosis were 0.773 （95% CI 0.776-0.841）,0.859 （95% CI 0.805-0.914）,and 0.796 （95% CI 0.726-0.866）,respectively.These three microRNAs were on a statistically significant elevation in GC patients compared with healthy controls at each of the four stages.However,there were no significant differences in the plasma levels of the three microRNAs among the four TNM stages （P 〉 0.05）.Conclusions Plasma miR-106b,miR-20a,and miR-221 may be used as biomarkers for the detection of early GC.
出处
《中华普通外科杂志》
CSCD
北大核心
2014年第2期115-118,共4页
Chinese Journal of General Surgery
基金
甘肃省科技支持计划项目资助
