摘要
目的:研究MMP-9的转录及表达与大肠癌的临床病理参数间的关系,探讨其在大肠癌浸润转移中的作用及机制。方法:采用定量逆转录聚合酶链反应和免疫组织化学方法,检测41例大肠癌标本及远癌切端结肠组织中MMP-9基因的mRNA转录水平及蛋白表达水平。结果:大肠癌中MMP-9的转录及表达水平均显著高于远癌切端正常组织(P<0.01),与大肠癌的浸润深度、Dukes分期及淋巴结转移密切相关(P<0.05)。结论:MMP-9在大肠癌的浸润转移中发挥重要作用,有助于判断患者预后情况。
Objective： To study the transcription level and expression level of MMP-9 in colorectal cancer and explore its function and the mechanism during the invasion and metastasis. Methods： The quantitative-RT-PCR and immunohistochemical method were employed to determine the transcription level and expression level of MMP-9 in 41 cases of colorectal cancer, including the cancer tissue and the distal cut margin. Results： Both the transcription level and expression level of MMP-9 in the cancer tissue were significantly higher than in the distal normal tissue. And the results were correlated with the invasion depth, lymph node metastasis and Dukes stage. Conclusion： MMP-9 plays an important role in the invasion and metastasis of colorectal cancer and is heloful for the judgment of prognosis.
出处
《天津医科大学学报》
2006年第3期406-408,共3页
Journal of Tianjin Medical University
关键词
大肠癌
浸润
转移
MMP-9
Colorectal cancer
Invasion
Metastasis
MMP-9
