摘要
目的探讨miR-29c-5p在胆囊癌组织中的表达情况及其临床意义。方法采用实时定量PCR技术（stem-loop real-time reverse transcription-polymerase chain reaction, qRT-PCR）检测40例胆囊癌组织与癌旁组织中miR．29c．5p的相对表达量；分析miR-29c．5p表达与胆囊癌患者临床病理因素及预后的关系。结果与癌旁组织相比，胆囊癌组织中miR-29c-Sp表达明显降低（Z=-2．521，P=0．008）．其表达与胆囊癌患者的淋巴结转移有关（X2=15．140，P〈0．001），而与年龄、性别、肿瘤大小、分化程度、浸润深度及肿瘤TNM分期无关（均P〉0．05）。MiR-29c-5p低表达与高表达的患者中位生存率分别为7．2个月和20．2个月，1年及3年生存率分别为32．0％、16．0％和55．7％、33．4％，差异有统计学意义（P=0．040）。MiR-29c-5p表达量不是影响胆囊癌患者生存的独立风险因素（P=0．974）。结论MiR．29c-5p在胆囊癌组织中表达下调，并可能与胆囊癌的淋巴结转移有关；miR-29c-5p低表达提示预后不良。
Objective To investigate the expression of miR-29c-Sp in patients with gallbladder cancer （GBC） and to analyze the association of the expression with the clinicopathologic characteristics. Methods The expression levels of miR-29c-5p in 40 patients with GBC along with their matched adjacent tissues were detected by using stem-loop real-time reverse transcription-polymerase chain reaction （qRT- qPCR）. The relations of miR-29c-Sp expression with the clinicopathologic factors and prognosis of GBC patients were analyzed. Results Compared with tumor-adjacent tissue, the miR-29c-5p expression in GBC was significantly downregulated （ Z = - 2. 521, P = 0. 008 ）. The expression of miR-29c-5p was closely associated with lynphatic metastasis （X2 = 15. 140, P 〈 0. 001 ）, but irrelevant to age, sex, tumor size, differentiation, T stage and TNM stage （ all P 〉0. 05 ）. The average survival period of low and high expression of miR-29c-5p were 7.2 months and 20. 2 months. The one-year and three-year survival rate of patients with low expression of miR-29c-5p were significantly lower than those with high levels （32.0% , 16.0% vs 55.7%, 33.4% ） （ P =0. 040）. Low miR-29c-5p expression is not risk factor affecting the survival of GBC patients （P = 0. 974 ）. Conclusions The miR-29c-5p expression is downregulated in GBC tissue, which probably may be associated with the metastasis of GBC, and low miR-29c-Sp expression may predict an unfavorable outcome.
出处
《中华普通外科杂志》
CSCD
北大核心
2015年第9期715-718,共4页
Chinese Journal of General Surgery
基金
上海市科委基础研究重点基金资助项目（12JC1406700）
关键词
胆囊肿瘤
淋巴转移
基因表达
Gallbladder neoplasms
Lymphatic metastasis
Gene expression
