摘要
目的 建立离子色谱法测定转化糖注射液的含量.方法 分析柱：Dionex CarboPac PA10阴离子分离柱（4mm×250mm）;淋洗液：25mmol·L-1 NaOH;流速：1mL·min-1;柱箱温度：30℃;进样量：10μL;检测方式：电化学检测器.结果 果糖、葡萄糖分别在10.196μg·mL-1～101.96μg·mL-1、10.382μg·mL-1～103.82μg·mL-1 浓度范围内有良好的线性关系,平均回收率分别为99.50%、99.83%.结论 该方法简便,准确.
OBJECTIVE To establish an IC method for the determination of content for invert sugar injec- tion. METHODS The analytical column was Dionex CarboPac PA10 column （4mm × 250mm） and the leacheate was 25mmol ·L-1 sodium hydrate at a flow rate of lmL ·min-1. The temperature of column was 30~C. RESULTS There were good linear relationships for fructose and glucose in the range of （5. 125 ~ 15. 375） μg ·mL-1 ,5. 155 15. 465 ） μg · mL-1 respectively. The average recoveries were 99.50% and 99.83% respectively. CONCLUSION The method is simple and accurate.
出处
《海峡药学》
2014年第1期60-61,共2页
Strait Pharmaceutical Journal
