摘要
建立离子色谱-脉冲安培检测法检测桃胶中的糖类（鼠李糖、阿拉伯糖、半乳糖、葡萄糖、甘露糖）含量的方法,采用METROSEP CARB 1（150 mm×4.0 mm）分离柱,以10 mmol/L Na OH溶液在1.0 m L/min下洗脱样品溶液,总分析时间为22 min。鼠李糖、甘露糖线性范围为5.0-100.0μg/m L,阿拉伯糖、半乳糖、葡萄糖线性范围为2.0-60.0μg/m L,鼠李糖、阿拉伯糖、半乳糖、葡萄糖、甘露糖检出限分别为（进样量20μL,S/N=3）0.34、0.18、0.17、0.08、0.39μg/m L,样品加标回收率为92.0%-98.3%,使用样品溶液连续进样6次,测得5种糖RSD为2.25%-4.23%。此方法无需衍生、操作方便、方法快捷、检测灵敏度高,适用于桃胶类糖含量的检测。
The ion chromatographic method with pulsed amperometric detectorhad was developed for the determination of peach gum polysaccharide content （ rhamnose, arabinose, galactose, glucose, mannose）. The analytes were separated on a METROSEP CARB l （150 mm ×4.0 mm）anion exchange column with 10 mmol/L NaOH as mobile phase at the flow rate of 1.0 mL/min, and the total analysis time of 22 min.The linear range of rhamnose and mannose was 5.0 to 100.0 μg/mL,the linear range of arabinose,galactose,glucose line arrange was 2.0 to 60.0 μg/mhThe detection limits of rhamnose, arabinose, galactose, glucose and mannose （20 μL injection, S/N = 3） were 0.34,0.18,0.17,0.08,0.39 μg/mL, respectively.The recovery rates ranged from 92.0% to 98.3%, and RSDs of 5 polysaccharide contents ranged from 2.25% to 4.23% after 6 consecutive injections. Therefore, the proposed determination method was derivative-free, simple,fast, effective and sensitive,which facilitates the rapid detection and effective quality control of polysaccharide contents in peach gum.
出处
《食品工业科技》
CAS
CSCD
北大核心
2016年第5期298-301,307,共5页
Science and Technology of Food Industry
基金
国家自然科学基金(21277110)资助
关键词
离子色谱
脉冲安培检测器
桃胶
多糖
Ion chromatography
pulsed ampere detector
peach gum
polysaccharide
