摘要
目的:探讨不同加工方法对泸州山银花品质的影响,为泸州山银花加工方法的筛选提供依据。方法:采用阴干、晒干、真空干燥、远红外干燥、微波干燥、烘干6种方法加工山银花,对外观性状进行描述,采用高效液相色谱法测定山银花中新绿原酸、绿原酸、异绿原酸A、异绿原酸C的含量。色谱柱为Kromasil C18柱(250 mm×4.6 mm,5μm);流动相为乙腈-0.1%磷酸水溶液,梯度洗脱;检测波长326 nm;柱温30℃;流速1 mL·min-1;进样量10μL。结果:6种加工方法中,真空干燥样品中新绿原酸、绿原酸、异绿原酸A及异绿原酸C的含量分别为0.094%、2.982%、1.804%、0.255%,高于其他5种方法所得样品;外观颜色和油润度均比其他5种方法好。结论:真空干燥方法得到的样品外观品相好,测得的新绿原酸、绿原酸、异绿原酸A及异绿原酸C含量高,明显优于其他干燥方法。
To discuss the effects of different drying methods on officinal qualities of Flos Lonicerae. Methods ：The following six methods were used：drying in the shade, drying in the sun, vacuum drying, far infrared drying, microwave drying, and oven drying. External qualities were studied and the contents of neochlorogenic acid, chlorogenic acid, on a Kromasile C sochlorogenic acid A and isochlorogenic acid s column（250 mm ×4.6 mm,5 μm）at 30℃. C were determined by HPLC. HPLC was performed The composition of mobile phase was acetonitrile - 0. 1% phosphoric acid with gradient elution,the flow rate was 1 mL · min-1 ,and the injection volume was 10 μL. Results：The content of neochlorogenic acid, chlorogenic acid, isochlorogenic acid A and isochl9rogenic acid C in the vacuum drying sample was 0. 094% ,2. 982% , 1. 804% and 0. 255% respectively, which was obviously higher than samples dried by other five methods;the color and texture of the vacuum drying sample was also better. Conclusion： The appearance and contents of chlorogenic acids of the vacuum drying sample are better than those of the others, indicating that the vacuum drying method is obviously better than the others.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2013年第11期1984-1988,共5页
Chinese Journal of Pharmaceutical Analysis
基金
泸州市重点科技项目(泸市财企【2010】41号)
泸州医学院2011年青年基金(2012026)
关键词
干燥方法
真空干燥
新绿原酸
绿原酸
异绿原酸A
异绿原酸C
HPLC
微波干燥
远红外干燥
drying method
vacuum drying
neochlorogenic acid
chlorogenic acid
isochlorogenic acid A
isochloro- genic acid C
HPLC
far infrared drying
microwave drying
