摘要
目的:建立应用紫外光谱快速测定金银花提取物中5种有机酸含量的方法。方法:以HPLC法为参照,测定金银花提取物中绿原酸、咖啡酸、3,4-二咖啡酰奎宁酸、3,5-二咖啡酰奎宁酸、4,5-二咖啡酰奎宁酸的含量。在220～400 nm范围内扫描金银花提取物溶液的紫外吸收光谱,采用偏最小二乘法分别建立样品紫外吸收光谱与5种有机酸含量之间的校正模型,并对模型的预测能力进行考察。结果:5个校正模型对预测集样本的预测值与对照值的相关系数都在0.90以上,相对预测误差分别为1.46%,-2.13%,0.42%,6.40%,3.83%,预测结果准确。结论:本方法操作简便、快速准确,可作为金银花提取物中有机酸含量测定的一种快速分析方法。
Objective： A UV method was established for the rapid determination of 5 kinds of organic acids in Flos Lonicerae extracts.Methods：The HPLC method was used as the reference method to determine the contents of chlorogenic acid,caffeic acid,3,4-dicaffeoylquinic acid,3,5-dicaffeoylquinic acid and 4,5-dicaffeoylquinic acid in the Flos Lonicerae extracts.UV absorption spectrum was scanned at the 220-400 nm wavelength range.The PLS models was developed to correlate the UV spectra with the corresponding values determined by the reference method.Then the performance of calibration models was validated.Result：The correlation coefficients of the validation set were all above 0.90,and the relative predicted errors for the 5 kinds of organic acid were 1.46%,-2.13%,0.42%,6.40% and 3.83%,respectively.Conclusion： Owing to its simple,fast and accurate properties,the presented method could be a method for the rapid determination of organic acids in Flos Lonicerae extracts.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2011年第3期547-551,共5页
Chinese Journal of Pharmaceutical Analysis
基金
国家重大科技专项"重大新药创制"(2009ZX09313-036)
