摘要
目的分析比较广西不同产地、不同加工方法山银花中绿原酸含量。方法采用高效液相色谱法（HPLC）进行测定。色谱柱为ODS-2 HYPERSIL（250mm×4．6mm，5μm），流动相为乙腈-0．4％磷酸（12：88），检测波长327nm。结果绿原酸在0．25—2μg范围内线性关系良好（r=0．99990），样品平均回收率为100.6％，RSD=2．98％（n=6）。结论南宁九塘、罗城怀群乡、罗城龙岸乡、融水杆桐乡的山银花绿原酸的含量较高；以脱水加工的山银花中绿原酸含量最高，蒸干、熏硫的次之。
Objective To compare the chlorogenic acid content of Flos Lonicerae with different origins, different processing methods. Methods Experiments were performed on ODS -2 HYPERSIL column （250 mm × 4.6 mm,5μm）. The mobile phase was acetonitrile -0.4% H3PO4 （12：88） , and the detection wavelength was 327nm. Results The standard curve of chlorogenic acid was linear with the regression coefficient of 0. 999 90 in the testing range of 0.25 - 2μ g. The average recovery was 100.6% ,with RSD of 2.98% （n = 6）. Conclusion Content of ehlorogenic acid in Flos Lonicerae from Jiutang, Huaiqun of Luocheng, Longan of Luocheng,Rongshui of Liuzhou was relatively higher. Chlorogenic acid in dehydrated Flos lnicerae was relatively higher, the steamed and the one smoked with sulphur followed.
出处
《时珍国医国药》
CAS
CSCD
北大核心
2008年第6期1370-1371,共2页
Lishizhen Medicine and Materia Medica Research
基金
广西科技厅科技攻关项目(No.桂科攻0424008-2D)
