摘要
目的建立血浆中玉米多糖铁（cornpolysaccharideironcomplex，CPIC）浓度的测定方法，并对玉米多糖铁在大鼠体内的吸收、分布和排泄过程进行研究。方法SD大鼠灌胃（28、14、7mg／kg）给予59Fe-玉米多糖铁后，采用放射性同位素示踪法测定不同时间点大鼠血浆中59Fe-玉米多糖铁的放射性，所得数据用DAS软件求算药动学参数并分析59Fe-玉米多糖铁在大鼠体内的组织分布和排泄。结果当59Fe-玉米多糖铁中铁离子的浓度在0．14～141μg／ml的浓度范围内时，其线性关系良好，r=0．9999（n=5）。平均回收率达到95％以上、RSD值低于15％。其主要药代动力学参数在三个剂量组的t1、2分别是214±104、231±110、181±81min，AUC（0-∞）分别为1986．3±513．3、737．0±467．0、315．1±226．1mg·min^-1·L^-1。在所测的13种组织中均有分布，但在胃肠道和造血器官及血流丰富器官中放射性浓度最高。大鼠口服59Fe-玉米多糖铁后主要通过粪便排出体外。结论用同位素示踪法测定血浆中59Fe-玉米多糖铁浓度，方法简单，专属性强，药动学参数表明其在大鼠体内的药动学模型符合二室模型。
Objective To establish a method for detecting plasma concentration of corn polysaccharide iron complex （CPIC） and investigate its absorption, distribution and elimination in rats. Methods Using radioactivity isotope tracing method, we detected the radioactivity of 59Fe-CPIC in the plasma of rats at different time points by garages of 3 doses （28.0, 14.0, and 7.0 mg/kg） 59Fe-CPIC in SD rats. The pharmacokinetic parameters was obtained using DAS 2.0 program for analysis of tissue distribution and elimination of 59Fe-CPIC. Results The standard curve was linear within the range of 0.14-141 gg/ml （r=0.9999, n=5）. The average recovery was 95% with a relative standard deviation no more than 15%. The pharmacokinetic parameters at 3 doses obtained, namely t1/2 and AUC （0-∞）, were 214±104, 231±110, and 181±81 min, and 1986.3±513.3, 737.0±467.0, and 315.1±226.1 mg·min·^-1· L^-1, respectively. 59Fe-CPIC were detected in all the 13 tissues types examined and high radioactivity intensity was found in the gastrointestinal tract, hematogenic organs and other organs rich in blood. 59Fe-CPIC was eliminated after intragastric administration primarily via the feces in rats. Conclusion The method we established is easy and specific, and the pharmacokinetic parameters of 59Fe-CPIC fit the two- compartment open model.
出处
《南方医科大学学报》
CAS
CSCD
北大核心
2013年第11期1638-1642,共5页
Journal of Southern Medical University
基金
安徽省教育厅自然科学基金(KJ2012B206)
