摘要
背景频域光学相干断层扫描（SD—OCT）可进行活体组织的测量。目前SD—OCT对人眼活体组织测量的研究已有较多报道，但对实验动物眼的活体测量结果少有研究。目的应用SD—OCT活体观察正常C57BL／6小鼠的眼前节结构及色素兔的角膜、视盘及视网膜形态结构。方法利用轴向分辨率为5μm、扫描速度为26000次／s的SD—OCT对4只健康SPF级C57BL／6小鼠8只眼扩瞳前后进行眼前节形态学检查；利用SD—OCT对6只健康SPF级色素兔12只眼进行角膜及视盘、视网膜形态学检查。结果SD—OCT进行眼前节扫描，清晰可见C57BL／6小鼠角膜、虹膜及瞳孔区对应的晶状体结构，而且扩瞳前后晶状体结构形态发生明显改变，角膜SD—OCT扫描断层图与相应的切片图结构相对应，扩瞳前平均中央角膜厚度（CCT）为（96±9）μm，平均前房深度（ACD）为（460-e8）汕m，平均角膜水平直径（wTw）为（2．86±0．41）mm；扩瞳后CCT为（96±8）μm，ACD为（356±20）μm，wTW为（2．87±0．62）mm，C57BL／6小鼠扩瞳前后CCT、wTw测量值的比较差异均无统计学意义（t=0．478，P=0．647；t=0．737，P=0．485）；扩瞳后ACD较扩瞳前明显变浅，差异有统计学意义（t=-13．022，P〈0．001）。色素兔的SD—OCT眼前节检查均可见明显的角膜及视网膜分层，结构分别与相应的组织学切片相对应，扩瞳后角膜最薄点均值为（370±10）μm，视网膜厚度均值为（175：e4）Ixm，水平扫描后手动测量数据视盘深度均值为（1．35±0．51）mm，宽度均值为（4．52±0．82）mm。结论SD—OCT作为一种非接触、非侵人性检查，可清晰呈现C57BL／6小鼠眼前节结构及色素兔相应的角膜和视网膜结构，测量指标可用于实验研究过程中相应组织结构的活体观察。
Background Spectral-domain optical coherence tomography （SD-OCT） can quantitatively analyze some ocular parameters in vivo. Although human ocular parameters have been obtained by SD-OCT, few studies were performed in animal experiment. Objective This study was to investigate the anterior and posterior segment parameters of C57BL/6 mice and pigmented rabbits using SD-OCT in vivo. Methods Some anterior and posterior segment ocular parameters,including the central corneal thickness （CCT） ,anterior chamber depth （ACD） , white-to-white （WTW）,optic nerve head （ONH） depth/width and retinal thickness, were measured in 8 eyes of 4 health SPF C57BL/6 mice and 12 eyes of 6 health SPF pigmented rabbits using SD-OCT. Results For C57BL/6 mice, Cornea,iris,lens in pupil area were clearly exhibited by SD-OCT. Mean CCT, ACD and WTW were （96±9）μm （460±8） μm and （ 2.86 ± 0. 41 ）mm pre-mydriasis, respectively, the corresponding values of post-mydriasis were （96＋8）μm,（356±20） μm and （2.87±0.62）ram. There were no statistical differences of CCT and WTW between pre- and post-mydriasis （ t = 0. 478, P = 0. 647 ; t = - 0. 737, P = 0. 485 ）. ACD of post-mydriasis was significantly shallower than that of baseline （t =- 13. 022, P〈0. 001 ）. For the pigmented rabbits, the thickness of corneal thinnest point,retinal thickness, ONH depth and width were （370 ± 10）mm, （175 ＋4） μm, （1.35 ±0.51） mm and （4.52±0.82） mm,respectively. Conclusions As a non-contact and non-invasive technology,SD-OCT can provide not only high resolution cross-sectional ocular images,but also high precise quantitative parameters for both C57BL/6 mouse and pigmented rabbit in vivo.
出处
《中华实验眼科杂志》
CAS
CSCD
北大核心
2013年第11期1055-1059,共5页
Chinese Journal Of Experimental Ophthalmology
