摘要
目的利用自行搭建的超长扫描深度的频域光学相干断层扫描（SD-OCT）活体测量小鼠眼球的眼轴长度、曲率等各眼内生物学参数，并评估其重复性和再现性。方法实验研究。本研究自主研发的超长扫描深度SD—OCT扫描深度7．3mm（空气中），系统轴向扫描（A—Scan）速度28kHz，轴向分辨率7．5um，横向分辨率19um。共有5只40日龄的C57BL/6小鼠（10眼）经历2天次的测量。第l天，由操作者1先执行测量步骤，连续拍摄2幅图后，由操作者2紧接着拍摄1幅图。在3次拍摄中，每次都保证前后拍摄的时间耽搁小于5min。2d以后．所有的小鼠再由操作者1拍摄1幅图，随后每只小鼠再进行1次时域OCT（TD—OCT）拍摄。所有测量都在下午2：00到4：00之间进行。测量的重复性和再现性评估都采用重复性系数、组内相关系数ICCs、Wilcoxon配对测试及绘制Bland．Altman图来综合分析。SD—OCT和TD．OCT的测量结果比较采用独立样本t检验。结果用SD—OCT成功获得了清晰、高质量的小鼠跟球一次性成像。除角膜、前房外，其他数据接近于TD—OCT。前房、晶状体、玻璃体和眼轴的重复性／再现性系数均小于3％且对应的组内相关系数（ICCs）几乎都大于0．75，角膜和视网膜的重复性／再现性系数小于5％且对应的ICCs相对较小。除操作者l第1天获取的2组角膜数据之间差异有统计学意义外（压一2-310，P〈0．05），其余差异均无统计学意义。眼轴等生物学参数的Bland．Ahman分析图显示所有的数据点都分布在95％可信区间内。各个曲率的重复性，再现性系数均小于5％。角膜前表面曲率半径和晶状体前、后表面曲率半径的ICCs基本大于0．75，而角膜后表面曲率半径的ICC大于0．45。Bland—Altman图显示曲率再现性的数据点约有5％分布在95％可信区间外。结论利用SD—OCT，可获得高清晰的从小鼠角膜到视网�
Objective To assess the accuracy, precision, repeatability, and reproducibility of biometric measurement of the mouse eye made by extended scan depth spectral domain optical coherence tomography （SD-OCT）. Methods It was an experimental study. An SD-OCT with an extended depth range of 7.3 mm, A-scan speed of 28 kHz, axial resolution of 9.0 um and transversally 19 um was developed. Five 40-day-old C57BL/6 mice （10 eyes） were imaged with a custom built, extended scan depth SD-OCT in 2 sessions. Two scans were performed by operator 1 and a following scan by operator 2 at the first session, to determine repeatability and interoperator reproducibility. Intersession reproducibility was assessed by an additional scan performed by operator 1 2 days later. The data were compared to those from time-domain OCT （TD-OCT） by independent t test. All experiments were performed between 2：00 PM and 4：00 PM. The repeatability and reproducibility were evaluated by coefficient of repeatability/reproducibility, intraclass correlation coefficients （ICCs）, Wilcoxon matched-pairs test and Bland-Altman graph. Results High-quality OCT images of the entire mouse eyes with high resolution were acquired. The ocular dimensions from TD-OCT measurements were close to these obtained by SD-OCT. The overall coeffieients of repeatability/reproducibility for anterior chamber depth, lens thickness, vitreous chamber and axial length were all less than 3% and relevant intraclass correlation coefficients （ICCs） were nearly greater than 0.75, whereas these were less than 5% and ICCs were smaller for corneal thickness and retinal thickness. Wileoxon analysis showed there was no statistically significant difference between any 2 groups for all parametres except corneal thickness of repeatability （Z=-2.310, P〈0.05）. From the graph of differences against mean for the axial lengths, it can be seen that 100% of the values fall within 2 SDs of the mean. The overall coefficients of repeatability/reproducibility for curvatures were les
出处
《中华眼视光学与视觉科学杂志》
CAS
2013年第6期347-353,共7页
Chinese Journal Of Optometry Ophthalmology And Visual Science
关键词
体层摄影术
光学相干
生物学测量
曲率
小鼠
Tomography,optical coherence
Biometric measurement
Curvature
Mice
