摘要
目的采用柱前衍生高效液相色谱法测定克拉霉素血药浓度。方法克拉霉素血浆样品以正己烷-正丁醇混合溶液萃取后,与9芴甲氧羰酰氯(FMOC-CL)衍生化后进样。色谱柱为Hypersil^(TM)ODS2柱(4.6mm×250mm,5μn),柱温:62℃;流动相:0.05mol/LNH_4H_2PO_4缓冲液(PH6.72)-乙腈=38:62(v/v),流速2.5 mL/min:激发波长:265m,发射波长:315nm。结果克拉霉素血药浓度在50~3000ng/mL范围内,浓度与峰面积比有良好的线性关系,最低检测浓度为30ng/mL,平均回收率为97.9~100.2%,日内RSD≤4.1%,日间RSD≤6.8%。结论本方法简单、快速、准确,可用于克拉霉素临床药动学研究。
Objective To establish a HPLC method with pre-column derivatization to measure the plasma concentration of clarithromycin. Methods A n-hexane and n-butanol extract of plasma was treated with FMOC-CL and chromatographed on a HypersilTM ODS2 column （4.6mm×250mm, 5μm） using an eluent composed of acetonitrile- 0.05mol/L phosphate buffer （PH6.72） （62：38）. The column temperature was at 62~C and flow rate of eluent was 2.5mL/min. The exciation and emission wavelength were 265nm and 315nm, respectively. Results The assay procedure was shown to produce linear calibration curves over the range 50ng/mL to 3000ng/mL of clarithromycin in plasma. The limit of detection was 30ng/mL. The recovery rate was 97.9～100.2%. The coefficients of variation within a day and between days did not exceed 4.1%, 6.8%, respectively. Conclusion This method is found to be easy, convenient and precise for clinical pharmacokinetics study of clarithromycin.
出处
《中国药物经济学》
2013年第5期22-24,共3页
China Journal of Pharmaceutical Economics
基金
广东省重大科技专项项目(2011A080300004)
