摘要
目的探讨光动力疗法对口腔生物膜致龋变形链球菌抑制作用。方法选择40颗新鲜健康恒牙标本制备成釉质块,随机分为4组每组10块釉质块,依此置入人工唾液孵育形成人工获得性膜,再置入变形链球菌液孵育人工致龋,单纯HMME组,单纯使用20μg/mL HMME孵育;单纯激光组,单纯采用激光照射;光动力组,20μg/mL HMME溶液孵育后进行再激光照射;阴性对照组,采用生理盐水处理。检测菌斑生物膜变形链球菌菌落数及抗菌率、变形链球菌代谢产物、人工龋釉质块表面显微硬度评价各组抗菌作用及对釉质质量的影响。结果各组菌落数和抗菌率差异均有统计学意义(P<0.05),光动力组菌落数最少,抗菌率最高。各组终末pH值和△pH值差异均有统计学意义(P<0.05),光动力组终末pH值最高,△pH值最小。各组人工龋釉质块表面显微硬度差异有统计学意义(P<0.05),光动力组人工龋釉质块表面显微硬度最高。结论光动力疗法对人工龋模型生物膜致龋变形链球菌抑制作用明显,抑制变形链球菌产生代谢酸,保护牙釉质提高抗龋能力,是一种有效的防龋方法,具有广泛的应用前景。
Objective To investigate the photodynamic therapy of oral biofilm cariogenic streptococcus mutans inhibi- tion. Methods A total of 40 fresh and healthy permanent teeth were prepared enamel blocks were randomly divided into four groups of 10 enamel blocks, and so placed in artificial saliva incubating artificial acquired pellicle, and then placed in the streptococcus mutans incubation in artificial the caries, simple HMME group, simply incubated with 20μg/mL HMME; laser alone group, simply using a laser irradiation; photodynamic groups, 20 μg/mL HMME solution after incubation, and then laser irradiation; negative control group using normal saline. Detection of plaque biofilm streptococcus mutans number of colonies and antibacterial rate, the streptococcus mutans metabolites, block of artifi- cial caries enamel surface microhardness evaluation of antibacterial effect and the quality of the enamel. Results The number of colonies and antibacterial rate differences were statistically significant （P 〈 0.05）, the least number of colonies of photodynamic group, the highest antibacterial rate. Terminal pH and delta pH value differences were statis tically significant （P 〈 0.05）, photodynamic terminal pH value of the highest minimum △pH value. Artificial caries enamel blocks microhardness, the difference was statistically significant （P 〈 0.05）, photodynamic group artificial caries highest surface microhardness of the enamel blocks. Conclusion Photodynamic therapy degree artificial caries model biofilm cariogenic streptococcus mutans significantly inhibited, inhibition of streptococcus mutans metabolic acid, protect the enamel to improve the ability of anti-caries, is an effective anti-caries has broad application prospects.
出处
《中国现代医生》
2013年第30期21-23,共3页
China Modern Doctor
基金
国家自然科学基金(81060312)
关键词
光动力疗法
口腔生物膜
人工致龋
变形链球菌
光敏剂
Photodynamic therapy
Oral biofilm
Artificial cariogenic
Streptococcus mutans
Photosensitizer
