摘要
目的 将通体发光光纤应用于光动力疗法（PDT）防龋,探讨其对大鼠磨牙牙釉质中Ca、P含量的影响.方法 接种变形链球菌（S.mutans）制备大鼠致龋模型.将80只Wistar大白鼠随机分成5组：17mW（8 mW/cm2）PDT组（A组）、34 mW（15 mW/cm2）PDT组（B组）、68 rnW（30 mW/cm2）PDT组（C组）、20 g/LNaF溶液组（阳性对照组,D组）和生理盐水组（阴性对照组,E组）,每组16只.采用650 nm半导体激光器,以质量浓度为40 μg/ml的血卟啉单甲醚为光敏剂,连续进行4周实验.应用电感耦合等离子体发射光谱法检测各组大鼠磨牙牙釉菌中的Ca、P含量.结果 实验后B、C、D组Ca、P含量明显高于A、E组,差异均具有统计学意义（均P＜0.05）.A组Ca、P含量实验前后差异均无统计学意义（均P＞0.05）;实验后B、C组Ca、P含量分别高于实验前,差异均具有统计学意义（均P＜0.05）.A组Ca增量低于D组,差异具有统计学意义（P＜0.05）;B、C两组Ca、P增量明显高于D组,差异均具有统计学意义（均P＜0.05）;而B、C两组间Ca、P增量差异均无统计学意义（均P＞0.05）.结论 在设定的参数范围内,PDT促进牙齿再矿化效果优于20 g/L NaF溶液.采用PDT防龋可增加大鼠磨牙牙釉质中的Ca、P含量,且Ca、P含量与PDT的功率有关.当PDT功率较低时,对釉质再矿化作用不明显;随着PDT功率的增加,Ca、P含量亦增加;当功率增加至一定数值时,两元素含量增量变化不明显,表明PDT可维持牙齿再矿化微环境.
Objective To study the application of photodynamic therapy （PDT） for dental caries prevention using whole body luminescence fiber,and to investigate the effects of PDT on the content of Ca and P in rat molar enamel.Method The rat dental caries model was established by inoculating with S.mutans.Eighty male rats were randomly divided into five groups,including three experimental groups：17 mW （8 mW/cm2） PDT （group A）,34 mW （15 mW/cm2） PDT （group B）,68 mW （30 mW/cm2） PDT （group C）,a positive control group：20 g/L NaF solution （group D）,and a negative control group：0.9% physiological saline （group E）.The experimental groups were treated by 40 μg/mL hematoporphyrin monomethyl ether （HMME） and 650 nm diode laser irradiation.The experiments were conducted for 4 weeks.The contents of Ca and P in the molars of each group were measured by inductively coupled plasma emission spectrometry.Results The contents of Ca and P in group B,C and D after PDT were significantly higher than those in group A and E （all P〈0.05）.The contents of Ca and P in group A showed no significant difference before and after PDT,while those in groups B and C showed significant increase after PDT （all P〈O.05）.The increment of Ca in group A after PDT was lower than that in group D （P〈0.05）,while those in group B and C were significantly higher than those in group D （all P〈0.05）.There was no significant difference in the increment of Ca and P between group B and C after PDT.Conclusions In the range of the experimental parameters,the PDT promoted effect of tooth remineralization is better than 20 g/L NaF.The levels of Ca and P in the tooth enamel can be promoted by PDT treatment,and the contents of Ca and P are related to the pewer of PDT.The effect of low power PDT on the remineralization of enamel is not obvious.The contents of Ca and P in the tooth enamel are increased with laser power of PDT.When the laser power increased to a certain value,the change in contents of the two elements
出处
《国际生物医学工程杂志》
CAS
2017年第2期123-127,共5页
International Journal of Biomedical Engineering
关键词
光动力疗法
微量元素
电感耦合等离子体发射光谱
再矿化
Photodynamic therapy
Trace elements
Inductively coupled plasma optical emission spectrometer
Remineralization
