摘要
目的了解耐甲氧西林金黄色葡萄球菌(MRSA)对不同消毒剂的抗性,探讨合理的消毒剂使用方案。方法以头孢西丁纸片扩散法(K-B法)筛选MRSA菌株,检测70%乙醇、0.5%氯己定醇、10%碘伏、0.5%氯已定对MRSA的最小抑菌浓度(MIC),比较MIC差异,并进行定量的杀菌试验检测。结果氯已定、碘伏、氯已定醇、乙醇的MIC50分别为64.0、64.0、2.4、1.0μg/mL;在30 s内,乙醇对MRSA的杀菌率在90%以上,在2 min内,乙醇可清除MRSA。结论 MRSA对氯已定、碘伏有抗性,对氯已定醇和乙醇敏感。
Objective To investigate the degree of resistance of different disinfectants on methicillin--resist- ant Staphylococcus aureus （MRSA）, in order to explore the rational use of disinfectants. Methods Cefoxitin disk diffusion （KB method） was applied to screen MRSA strains; the minimum inhibitory concentration （MIC） of 70% ethanol, 0.5 % chlorhexidine alcohol, 10% povidone-iodine, and 0.5 chlorhexidine against MRSA were tested; the MIC differences were compared; quantitative germicidal test of disinfectants were conducted. Results The MICs0 of chlorhexidine, povidone-iodine, chlorhexidine alcohol and ethanol were 64.0 μg/mL, 64.0 μg/mL, 2.4 μg/mL and 1.0 μg/mL, respectively. In 30 seconds, the bactericidal rate of ethanol against MRSA was 90%. In 2 minutes, the rate was 100%. Oonclusion MRSA is resistant to chlorhexidine and povidone-iodine while sensitive to chlorhexidine and ethanol,
出处
《国外医学（医学地理分册）》
CAS
2013年第3期184-186,190,共4页
Foreign Medical Sciences：Section of Medgeography
关键词
耐甲氧西林金黄色葡萄球菌
消毒剂
抗性
methicillin resistant Staphylococcus aureus
disinfectant
resistance
