摘要
目的本研究旨在探讨6种消毒剂对沙门氏菌和大肠杆菌的抑菌效果,明确细菌对消毒剂的耐受效果,为实验动物屏障环境科学使用消毒剂提供重要依据。方法选用分离出的沙门氏菌和大肠杆菌,采用滤纸片法测定各种消毒剂的抑菌、杀菌效果。结果实验结果显示0.05%古迪安对沙门氏菌和大肠杆菌的抑菌作用效果显著高于其他消毒剂(P<0.05),其次是0.05%百毒杀和0.25%戊二醛溶液,0.2%过氧乙酸、0.1%二氯异氰脲酸钠粉和0.25%新洁尔灭抑菌效果较差。结论本实验0.05%古迪安对沙门氏菌和大肠杆菌的杀菌效果最有效,可为今后使用动物屏障环境消毒提供重要参考。
Objective To investigate the antibacterial effect of six kinds of disinfectants on Salmonella and Escherichia coli,to determine the tolerance of bacterias to disinfectants,and to provide important evidence for the scientific use of disinfectants in the barrier environment of laboratory animals.Method Salmonella and Escherichia coli were isolated and the bacteriostatic and bactericidal effects of various disinfectants were determined by filter paper method.Result The result showed that 0.05%Goudian had significantly higher bacteriostatic effect on Salmonella and Escherichiacoli than other disinfectants(P<0.05),followed by 0.05%Baidu and0.25%glutaraldehyde solution,0.2%peracetic acid,0.1%sodium dichloroisocyanurate powder and 0.25%neogel.Conclusion In this experiment,0.05%Goudian is the most effective disinfectant for Salmonella and Escherichiacoli,which can provide important reference for the future use of animal barrier environment disinfection.
作者
梁锦宁
李家富
欧阳轶强
张名媛
孙俊铭
郭晓萍
LIANG Jinning;LI Jiafu;OUYANG Yiqiang;ZHANG Mingyuan;SUN Junming;GUO Xiaoping(Guangxi Medical University Laboratory Animal Center,Nanning 530021,China)
出处
《实验动物科学》
2020年第1期38-41,共4页
Laboratory Animal Science
关键词
消毒剂
沙门氏菌
大肠杆菌
抑菌效果
disinfectant
Salmonella
Escherichia coli
antibacterial effect
